Title |
Selective Elimination of Mitochondrial Mutations in the Germline by Genome Editing
|
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Published in |
Cell, April 2015
|
DOI | 10.1016/j.cell.2015.03.051 |
Pubmed ID | |
Authors |
Pradeep Reddy, Alejandro Ocampo, Keiichiro Suzuki, Jinping Luo, Sandra R. Bacman, Sion L. Williams, Atsushi Sugawara, Daiji Okamura, Yuji Tsunekawa, Jun Wu, David Lam, Xiong Xiong, Nuria Montserrat, Concepcion Rodriguez Esteban, Guang-Hui Liu, Ignacio Sancho-Martinez, Dolors Manau, Salva Civico, Francesc Cardellach, Maria del Mar O’Callaghan, Jaime Campistol, Huimin Zhao, Josep M. Campistol, Carlos T. Moraes, Juan Carlos Izpisua Belmonte |
Abstract |
Mitochondrial diseases include a group of maternally inherited genetic disorders caused by mutations in mtDNA. In most of these patients, mutated mtDNA coexists with wild-type mtDNA, a situation known as mtDNA heteroplasmy. Here, we report on a strategy toward preventing germline transmission of mitochondrial diseases by inducing mtDNA heteroplasmy shift through the selective elimination of mutated mtDNA. As a proof of concept, we took advantage of NZB/BALB heteroplasmic mice, which contain two mtDNA haplotypes, BALB and NZB, and selectively prevented their germline transmission using either mitochondria-targeted restriction endonucleases or TALENs. In addition, we successfully reduced human mutated mtDNA levels responsible for Leber's hereditary optic neuropathy (LHOND), and neurogenic muscle weakness, ataxia, and retinitis pigmentosa (NARP), in mammalian oocytes using mitochondria-targeted TALEN (mito-TALENs). Our approaches represent a potential therapeutic avenue for preventing the transgenerational transmission of human mitochondrial diseases caused by mutations in mtDNA. PAPERCLIP. |
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Mendeley readers
Geographical breakdown
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United States | 4 | <1% |
Switzerland | 2 | <1% |
Germany | 2 | <1% |
Chile | 1 | <1% |
Canada | 1 | <1% |
France | 1 | <1% |
Japan | 1 | <1% |
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Other | 0 | 0% |
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Student > Ph. D. Student | 97 | 18% |
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Other | 29 | 5% |
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Unknown | 77 | 14% |
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Chemistry | 9 | 2% |
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