| Title |
An Ixodes scapularis cell line with a predominantly neuron-like phenotype
|
|---|---|
| Published in |
Experimental and Applied Acarology, April 2015
|
| DOI | 10.1007/s10493-015-9908-1 |
| Pubmed ID | |
| Authors |
Jonathan D. Oliver, Adela S. Oliva Chávez, Roderick F. Felsheim, Timothy J. Kurtti, Ulrike G. Munderloh |
| Abstract |
The Ixodes scapularis embryo-derived cell line ISE6 is the most widely utilized tick-derived cell line due to its susceptibility to a wide variety of tick- and non-tick-vectored pathogens. Little is known about its tissue origin or biological background. Protein expression of ISE6 cells was compared with that of another I. scapularis-derived cell line, IDE12, and dissected tick synganglia. Results demonstrated the presence of a neuronal marker protein, type 3 β-tubulin, in all three samples, as well as other shared and unique neuronal and immune response-associated proteins. Of neuronal proteins shared between the two cell lines, ISE6 expressed several in significantly greater quantities than IDE12. Stimulation of ISE6 cells by in vivo exposure to the hemocoel environment in unfed larval and molting nymphal ticks, but not unfed nymphal ticks, resulted in the development of neuron-like morphologic characteristics in the implanted cells. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 39 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 15% |
| Researcher | 6 | 15% |
| Student > Doctoral Student | 3 | 8% |
| Student > Bachelor | 3 | 8% |
| Other | 3 | 8% |
| Other | 9 | 23% |
| Unknown | 9 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 26% |
| Biochemistry, Genetics and Molecular Biology | 7 | 18% |
| Unspecified | 2 | 5% |
| Computer Science | 2 | 5% |
| Engineering | 2 | 5% |
| Other | 5 | 13% |
| Unknown | 11 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 April 2015.
All research outputs
#19,246,640
of 23,849,058 outputs
Outputs from Experimental and Applied Acarology
#620
of 914 outputs
Outputs of similar age
#195,937
of 266,769 outputs
Outputs of similar age from Experimental and Applied Acarology
#15
of 19 outputs
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