| Title |
13C-detected NMR experiments for automatic resonance assignment of IDPs and multiple-fixing SMFT processing
|
|---|---|
| Published in |
Journal of Biomolecular NMR, April 2015
|
| DOI | 10.1007/s10858-015-9932-9 |
| Pubmed ID | |
| Authors |
Paweł Dziekański, Katarzyna Grudziąż, Patrik Jarvoll, Wiktor Koźmiński, Anna Zawadzka-Kazimierczuk |
| Abstract |
Intrinsically disordered proteins (IDPs) have recently attracted much interest, due to their role in many biological processes, including signaling and regulation mechanisms. High-dimensional (13)C direct-detected NMR experiments have proven exceptionally useful in case of IDPs, providing spectra with superior peak dispersion. Here, two such novel experiments recorded with non-uniform sampling are introduced, these are 5D HabCabCO(CA)NCO and 5D HNCO(CA)NCO. Together with the 4D (HACA)CON(CA)NCO, an extension of the previously published 3D experiments (Pantoja-Uceda and Santoro in J Biomol NMR 59:43-50, 2014. doi: 10.1007/s10858-014-9827-1 ), they form a set allowing for complete and reliable resonance assignment of difficult IDPs. The processing is performed with sparse multidimensional Fourier transform based on the concept of restricting (fixing) some of spectral dimensions to a priori known resonance frequencies. In our study, a multiple-fixing method was developed, that allows easy access to spectral data. The experiments were tested on a resolution-demanding alpha-synuclein sample. Due to superior peak dispersion in high-dimensional spectrum and availability of the sequential connectivities between four consecutive residues, the overwhelming majority of resonances could be assigned automatically using the TSAR program. |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Poland | 1 | 4% |
| Unknown | 25 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 19% |
| Professor | 4 | 15% |
| Student > Ph. D. Student | 4 | 15% |
| Student > Bachelor | 3 | 12% |
| Other | 2 | 8% |
| Other | 4 | 15% |
| Unknown | 4 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 35% |
| Chemistry | 5 | 19% |
| Agricultural and Biological Sciences | 5 | 19% |
| Nursing and Health Professions | 1 | 4% |
| Unknown | 6 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 April 2015.
All research outputs
#18,407,102
of 22,800,560 outputs
Outputs from Journal of Biomolecular NMR
#461
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#193,450
of 265,380 outputs
Outputs of similar age from Journal of Biomolecular NMR
#5
of 13 outputs
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