| Title |
A Th1 cytokine–enriched microenvironment enhances tumor killing by activated T cells armed with bispecific antibodies and inhibits the development of myeloid-derived suppressor cells
|
|---|---|
| Published in |
Cancer Immunology, Immunotherapy, October 2011
|
| DOI | 10.1007/s00262-011-1116-1 |
| Pubmed ID | |
| Authors |
Archana Thakur, Dana Schalk, Sanila H. Sarkar, Zaid Al-Khadimi, Fazlul H. Sarkar, Lawrence G. Lum |
| Abstract |
In this study, we investigated whether activated T cells (ATC) armed with bispecific antibodies (aATC) can inhibits tumor growth and MDSC development in a Th1 cytokine-enriched (IL-2 and IFN-γ) microenvironment. Cytotoxicity mediated by aATC was significantly higher (P < 0.001) against breast cancer cell lines in the presence of Th1 cytokines as compared with control co-cultures. In the presence of aATC, CD33+ /CD11b+ /CD14- /HLA-DR- MDSC population was reduced significantly under both control (P < 0.03) and Th1-enriched (P < 0.036) culture conditions. Cytokine analysis in the culture supernatants showed high levels of MDSC suppressive chemokines CXCL9 and CXCL10 in Th1-enriched culture supernatants with highly significant increase (P < 0.001) in the presence of aATC. Interestingly, MDSC recovered from co-cultures without aATC showed potent ability to suppress activated T-cell-mediated cytotoxicity (P < 0.001), IFN-γ production (P < 0.01) and T-cell proliferation (P < 0.05) compared to those recovered from aATC-containing co-cultures. These data suggest that aATC can mediate enhanced killing of tumor cells and may suppress MDSC and T(reg) differentiation, and presence of Th() cytokines potentiates aATC-induced suppression of MDSC, suggesting that Th1-enriching immunotherapy may be beneficial in cancer treatment. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | 3% |
| Unknown | 37 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 26% |
| Researcher | 9 | 24% |
| Student > Bachelor | 5 | 13% |
| Student > Doctoral Student | 3 | 8% |
| Student > Master | 2 | 5% |
| Other | 3 | 8% |
| Unknown | 6 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 13 | 34% |
| Biochemistry, Genetics and Molecular Biology | 6 | 16% |
| Agricultural and Biological Sciences | 6 | 16% |
| Immunology and Microbiology | 4 | 11% |
| Veterinary Science and Veterinary Medicine | 2 | 5% |
| Other | 0 | 0% |
| Unknown | 7 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 October 2011.
All research outputs
#18,297,449
of 22,653,392 outputs
Outputs from Cancer Immunology, Immunotherapy
#2,423
of 2,882 outputs
Outputs of similar age
#110,429
of 132,933 outputs
Outputs of similar age from Cancer Immunology, Immunotherapy
#33
of 35 outputs
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