Title |
Cell Specific eQTL Analysis without Sorting Cells
|
---|---|
Published in |
PLoS Genetics, May 2015
|
DOI | 10.1371/journal.pgen.1005223 |
Pubmed ID | |
Authors |
Harm-Jan Westra, Danny Arends, Tõnu Esko, Marjolein J Peters, Claudia Schurmann, Katharina Schramm, Johannes Kettunen, Hanieh Yaghootkar, Benjamin P Fairfax, Anand Kumar Andiappan, Yang Li, Jingyuan Fu, Juha Karjalainen, Mathieu Platteel, Marijn Visschedijk, Rinse K Weersma, Silva Kasela, Lili Milani, Liina Tserel, Pärt Peterson, Eva Reinmaa, Albert Hofman, André G Uitterlinden, Fernando Rivadeneira, Georg Homuth, Astrid Petersmann, Roberto Lorbeer, Holger Prokisch, Thomas Meitinger, Christian Herder, Michael Roden, Harald Grallert, Samuli Ripatti, Markus Perola, Andrew R Wood, David Melzer, Luigi Ferrucci, Andrew B Singleton, Dena G Hernandez, Julian C Knight, Rossella Melchiotti, Bernett Lee, Michael Poidinger, Francesca Zolezzi, Anis Larbi, De Yun Wang, Leonard H van den Berg, Jan H Veldink, Olaf Rotzschke, Seiko Makino, Veikko Salomaa, Konstantin Strauch, Uwe Völker, Joyce B J van Meurs, Andres Metspalu, Cisca Wijmenga, Ritsert C Jansen, Lude Franke |
Abstract |
The functional consequences of trait associated SNPs are often investigated using expression quantitative trait locus (eQTL) mapping. While trait-associated variants may operate in a cell-type specific manner, eQTL datasets for such cell-types may not always be available. We performed a genome-environment interaction (GxE) meta-analysis on data from 5,683 samples to infer the cell type specificity of whole blood cis-eQTLs. We demonstrate that this method is able to predict neutrophil and lymphocyte specific cis-eQTLs and replicate these predictions in independent cell-type specific datasets. Finally, we show that SNPs associated with Crohn's disease preferentially affect gene expression within neutrophils, including the archetypal NOD2 locus. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 27% |
Italy | 1 | 7% |
Norway | 1 | 7% |
Unknown | 9 | 60% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 8 | 53% |
Scientists | 7 | 47% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 1% |
Netherlands | 3 | 1% |
Finland | 1 | <1% |
Germany | 1 | <1% |
Canada | 1 | <1% |
United Kingdom | 1 | <1% |
Denmark | 1 | <1% |
Mexico | 1 | <1% |
Unknown | 255 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 69 | 26% |
Researcher | 67 | 25% |
Student > Master | 24 | 9% |
Student > Bachelor | 20 | 7% |
Professor | 10 | 4% |
Other | 41 | 15% |
Unknown | 37 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 89 | 33% |
Biochemistry, Genetics and Molecular Biology | 69 | 26% |
Medicine and Dentistry | 19 | 7% |
Computer Science | 14 | 5% |
Immunology and Microbiology | 7 | 3% |
Other | 21 | 8% |
Unknown | 49 | 18% |