Title |
An RNA aptamer that specifically binds pancreatic adenocarcinoma up-regulated factor inhibits migration and growth of pancreatic cancer cells
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Published in |
Cancer Letters, September 2011
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DOI | 10.1016/j.canlet.2011.08.027 |
Pubmed ID | |
Authors |
Yun-Hee Kim, Ho Jin Sung, Sukyoung Kim, Eun-Ok Kim, Ji Won Lee, Ju Young Moon, Kyungho Choi, Ji-Eun Jung, Yangsoon Lee, Sang Seok Koh, Sue Goo Rhee, Kyun Heo, In-Hoo Kim |
Abstract |
Previously, we reported that a novel secretory protein, pancreatic adenocarcinoma up-regulated factor (PAUF), which is highly expressed in pancreatic cancer and mediates the growth and metastasis of pancreatic cancer cells. In this study, we generated and characterized a 2'-fluoropyrimidine modified RNA aptamer (P12FR2) directed against human PAUF. P12FR2 binds specifically to human PAUF with an estimated apparent K(D) of 77nM. P12FR2 aptamer inhibits PAUF-induced migration of PANC-1, human pancreatic cancer cells, in a wound healing assay. Moreover, intraperitoneal injection of P12FR2 decreased tumor growth by about 60% in an in vivo xenograft model with CFPAC-1 pancreatic cancer cells, without causing a loss of weight in the treated mice. Taken together, we propose here that PAUF-specific RNA aptamer, P12FR2, has the potential to be effective in the therapy of human pancreatic cancer. |
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United States | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 15 | 25% |
Researcher | 11 | 18% |
Student > Master | 8 | 13% |
Student > Bachelor | 4 | 7% |
Other | 3 | 5% |
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Biochemistry, Genetics and Molecular Biology | 8 | 13% |
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Medicine and Dentistry | 4 | 7% |
Other | 9 | 15% |
Unknown | 14 | 23% |