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X Demographics
Mendeley readers
Attention Score in Context
| Title |
A Leukemia-Associated CD34/CD123/CD25/CD99+ Immunophenotype Identifies FLT3-Mutated Clones in Acute Myeloid Leukemia
|
|---|---|
| Published in |
Clinical Cancer Research, August 2015
|
| DOI | 10.1158/1078-0432.ccr-14-3186 |
| Pubmed ID | |
| Authors |
Daniela F. Angelini, Tiziana Ottone, Gisella Guerrera, Serena Lavorgna, Michela Cittadini, Francesco Buccisano, Marco De Bardi, Francesca Gargano, Luca Maurillo, Mariadomenica Divona, Nélida I. Noguera, Maria Irno Consalvo, Giovanna Borsellino, Giorgio Bernardi, Sergio Amadori, Adriano Venditti, Luca Battistini, Francesco Lo-Coco |
| Abstract |
Purpose We evaluated leukemia-associated immunophenotypes (LAIP) and their correlation with fms-like tyrosine kinase 3 (FLT3) and nucleophosmin (NPM1) gene mutational status in order to contribute a better identification of patients at highest risk of relapse in AML. Bone marrow samples from 132 patients with acute myeloid leukemia (AML) were analyzed by 9-color multiparametric flow cytometry (MPFC). We confirmed the presence of the mutation in diagnostic samples and in sorted cells by conventional RT-PCR and by patient-specific RQ-PCR. Within the CD34+ve cell fraction we identified a discrete population expressing high levels of the IL-3 receptor alpha-chain (CD123) and MIC-2 (CD99) in combination with the IL-2 receptor alpha-chain (CD25). The presence of this population positively correlated with the internal tandem duplications (ITD) mutation in the FLT3 gene (r = 0.71). Receiver operating characteristics (ROC) showed that, within the CD34+ve cell fraction a percentage of CD123/CD99/CD25+ve cells ≥ 11.7% predicted FLT3-ITD mutations with a specificity and sensitivity of >90%. CD34/CD123/CD99/CD25+ve clones were also detectable at presentation in 3 patients with FLT3 wild-type/NPM1+ve AML who relapsed with FLT3-ITD/NPM1+ve AML. Quantitative real-time PCR designed at relapse for each FLT3-ITD in these three cases confirmed the presence of low copy numbers of the mutation in diagnostic samples. Our results suggest that the CD34/25/123/99+ve LAIP is strictly associated with FLT3-ITD positive cells. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 75% |
| Unknown | 1 | 25% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Scientists | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 72 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 72 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 17% |
| Student > Ph. D. Student | 11 | 15% |
| Student > Bachelor | 9 | 13% |
| Student > Doctoral Student | 6 | 8% |
| Professor | 4 | 6% |
| Other | 15 | 21% |
| Unknown | 15 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 30 | 42% |
| Biochemistry, Genetics and Molecular Biology | 10 | 14% |
| Agricultural and Biological Sciences | 7 | 10% |
| Immunology and Microbiology | 3 | 4% |
| Social Sciences | 1 | 1% |
| Other | 1 | 1% |
| Unknown | 20 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 July 2019.
All research outputs
#14,223,874
of 22,803,211 outputs
Outputs from Clinical Cancer Research
#9,921
of 12,588 outputs
Outputs of similar age
#138,076
of 266,605 outputs
Outputs of similar age from Clinical Cancer Research
#113
of 165 outputs
Altmetric has tracked 22,803,211 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 12,588 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.8. This one is in the 19th percentile – i.e., 19% of its peers scored the same or lower than it.
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