Title |
Proanthocyanidin-containing polyphenol extracts from fruits prevent the inhibitory effect of hydrogen sulfide on human colonocyte oxygen consumption
|
---|---|
Published in |
Amino Acids, April 2018
|
DOI | 10.1007/s00726-018-2558-y |
Pubmed ID | |
Authors |
Mireille Andriamihaja, Annaïg Lan, Martin Beaumont, Marta Grauso, Martin Gotteland, Edgar Pastene, Maria Jose Cires, Catalina Carrasco-Pozo, Daniel Tomé, François Blachier |
Abstract |
Hydrogen sulfide (H2S), a metabolic end product synthesized by the microbiota from L-cysteine, has been shown to act at low micromolar concentration as a mineral oxidative substrate in colonocytes while acting as an inhibitor of oxygen consumption at higher luminal concentrations (65 µM and above). From the previous works showing that polyphenols can bind volatile sulfur compounds, we hypothesized that different dietary proanthocyanidin-containing polyphenol (PACs) plant extracts might modulate the inhibitory effect of H2S on colonocyte respiration. Using the model of human HT-29 Glc-/+ cell colonocytes, we show here that pre-incubation of 65 µM of the H2S donor NaHS with the different polyphenol extracts markedly reduced the inhibitory effect of NaHS on colonocyte oxygen consumption. Our studies on HT-29 Glc-/+ cell respiration performed in the absence or the presence of PACs reveal rapid binding of H2S with the sulfide-oxidizing unit and slower binding of H2S to the cytochrome c oxidase (complex IV of the respiratory chain). Despite acute inhibition of colonocyte respiration, no measurable effect of NaHS on paracellular permeability was recorded after 24 h treatment using the Caco-2 colonocyte monolayer model. The results are discussed in the context of the binding of excessive bacterial metabolites by unabsorbed dietary compounds and of the capacity of colonocytes to adapt to changing luminal environment. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 8 | 30% |
Student > Master | 3 | 11% |
Student > Doctoral Student | 2 | 7% |
Student > Bachelor | 2 | 7% |
Student > Postgraduate | 2 | 7% |
Other | 3 | 11% |
Unknown | 7 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 15% |
Biochemistry, Genetics and Molecular Biology | 3 | 11% |
Medicine and Dentistry | 3 | 11% |
Nursing and Health Professions | 2 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Other | 4 | 15% |
Unknown | 10 | 37% |