| Title |
Efficient treatment of breast cancer xenografts with multifunctionalized iron oxide nanoparticles combining magnetic hyperthermia and anti-cancer drug delivery
|
|---|---|
| Published in |
Breast Cancer Research, May 2015
|
| DOI | 10.1186/s13058-015-0576-1 |
| Pubmed ID | |
| Authors |
Susanne Kossatz, Julia Grandke, Pierre Couleaud, Alfonso Latorre, Antonio Aires, Kieran Crosbie-Staunton, Robert Ludwig, Heidi Dähring, Volker Ettelt, Ana Lazaro-Carrillo, Macarena Calero, Maha Sader, José Courty, Yuri Volkov, Adriele Prina-Mello, Angeles Villanueva, Álvaro Somoza, Aitziber L Cortajarena, Rodolfo Miranda, Ingrid Hilger |
| Abstract |
Tumor cells can effectively be killed by heat, e.g. by using magnetic hyperthermia. The main challenge in the field, however, is the deposition of therapeutic temperatures selectively in the whole tumor region. We aimed to improve magnetic hyperthermia of breast cancer by using innovative nanoparticles which display a high heating potential and are functionalized with a cell internalization and a chemotherapeutic agent to increase cell death. The superparamagnetic iron oxide nanoparticles (MF66) were electrostatically functionalized with either Nucant multivalent pseudopeptide (N6L; MF66-N6L), doxorubicin (DOX; MF66-DOX) or both (MF66-N6LDOX). Their cytotoxic potential was assessed in a breast adenocarcinoma cell line MDA-MB-231. Therapeutic efficacy was analyzed on subcutaneous MDA-MB-231 tumor bearing female athymic nude mice. All nanoparticle variants showed an excellent heating potential around 500 W/g Fe in the alternating magnetic field (AMF, conditions: H = 15.4 kA/m, f = 435 kHz). We could show a gradual inter- and intracellular release of the ligands, and nanoparticle uptake in cells was increased by the N6L functionalization. MF66-DOX and MF66-N6LDOX in combination with hyperthermia were more cytotoxic to breast cancer cells than the respective free ligands. We observed a substantial tumor growth inhibition (to 40% of the initial tumor volume, complete tumor regression in many cases) after intratumoral injection of the nanoparticles in vivo. The proliferative activity of the remaining tumor tissue was distinctly reduced. The therapeutic effects of breast cancer magnetic hyperthermia could be strongly enhanced by the combination of MF66 functionalized with N6L and DOX and magnetic hyperthermia. Our approach combines two ways of tumor cell killing (magnetic hyperthermia and chemotherapy) and represents a straightforward strategy for translation into the clinical practice when injecting nanoparticles intratumorally. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 294 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 2 | <1% |
| Germany | 1 | <1% |
| India | 1 | <1% |
| Turkey | 1 | <1% |
| United States | 1 | <1% |
| Poland | 1 | <1% |
| Unknown | 287 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 53 | 18% |
| Student > Master | 48 | 16% |
| Researcher | 44 | 15% |
| Student > Bachelor | 31 | 11% |
| Student > Doctoral Student | 16 | 5% |
| Other | 31 | 11% |
| Unknown | 71 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 41 | 14% |
| Biochemistry, Genetics and Molecular Biology | 33 | 11% |
| Engineering | 27 | 9% |
| Materials Science | 22 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 21 | 7% |
| Other | 66 | 22% |
| Unknown | 84 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 11. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 February 2016.
All research outputs
#3,222,457
of 25,374,917 outputs
Outputs from Breast Cancer Research
#335
of 2,053 outputs
Outputs of similar age
#39,743
of 279,152 outputs
Outputs of similar age from Breast Cancer Research
#4
of 38 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. Compared to these this one has done well and is in the 87th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,053 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 12.2. This one has done well, scoring higher than 83% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,152 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 38 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.