Title |
Sequential backbone assignment based on dipolar amide-to-amide correlation experiments
|
---|---|
Published in |
Journal of Biomolecular NMR, May 2015
|
DOI | 10.1007/s10858-015-9945-4 |
Pubmed ID | |
Authors |
ShengQi Xiang, Kristof Grohe, Petra Rovó, Suresh Kumar Vasa, Karin Giller, Stefan Becker, Rasmus Linser |
Abstract |
Proton detection in solid-state NMR has seen a tremendous increase in popularity in the last years. New experimental techniques allow to exploit protons as an additional source of information on structure, dynamics, and protein interactions with their surroundings. In addition, sensitivity is mostly improved and ambiguity in assignment experiments reduced. We show here that, in the solid state, sequential amide-to-amide correlations turn out to be an excellent, complementary way to exploit amide shifts for unambiguous backbone assignment. For a general assessment, we compare amide-to-amide experiments with the more common (13)C-shift-based methods. Exploiting efficient CP magnetization transfers rather than less efficient INEPT periods, our results suggest that the approach is very feasible for solid-state NMR. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 3% |
Unknown | 32 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 13 | 39% |
Researcher | 11 | 33% |
Professor > Associate Professor | 2 | 6% |
Student > Master | 1 | 3% |
Student > Doctoral Student | 1 | 3% |
Other | 1 | 3% |
Unknown | 4 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 16 | 48% |
Biochemistry, Genetics and Molecular Biology | 10 | 30% |
Physics and Astronomy | 1 | 3% |
Unknown | 6 | 18% |