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RNA Scaffolds

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Cover of 'RNA Scaffolds'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 A Method to Predict the 3D Structure of an RNA Scaffold
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    Chapter 2 Post-crystallization Improvement of RNA Crystal Diffraction Quality
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    Chapter 3 Expression and Purification of RNA–Protein Complexes in Escherichia coli
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    Chapter 4 Production of Homogeneous Recombinant RNA Using a tRNA Scaffold and Hammerhead Ribozymes
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    Chapter 5 In Vivo Production of Small Recombinant RNAs Embedded in a 5S rRNA-Derived Protective Scaffold
  7. Altmetric Badge
    Chapter 6 Detection of RNA–Protein Interactions Using Tethered RNA Affinity Capture
  8. Altmetric Badge
    Chapter 7 A Universal Method for Labeling Native RNA in Live Bacterial Cells
  9. Altmetric Badge
    Chapter 8 Live Cell Imaging Using Riboswitch-Spinach tRNA Fusions as Metabolite-Sensing Fluorescent Biosensors.
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    Chapter 9 RNA Scaffold: Designed to Co-localize Enzymes
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    Chapter 10 Artificial Ligase Ribozymes Isolated by a “Design and Selection” Strategy
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    Chapter 11 Engineering aptazyme switches for conditional gene expression in Mammalian cells utilizing an in vivo screening approach.
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    Chapter 12 Aptazyme-Based Riboswitches and Logic Gates in Mammalian Cells
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    Chapter 13 Design and Characterization of Topological Small RNAs.
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    Chapter 14 Folding RNA-Protein Complex into Designed Nanostructures.
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    Chapter 15 Simple Method for Constructing RNA Triangle, Square, Pentagon by Tuning Interior RNA 3WJ Angle from 60° to 90° or 108°.
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    Chapter 16 RNA-Mediated CdS-Based Nanostructures.
  18. Altmetric Badge
    Chapter 17 An Effective Method for Specific Gene Silencing in Escherichia coli Using Artificial Small RNA.
Attention for Chapter 14: Folding RNA-Protein Complex into Designed Nanostructures.
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Chapter title
Folding RNA-Protein Complex into Designed Nanostructures.
Chapter number 14
Book title
RNA Scaffolds
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2730-2_14
Pubmed ID
Book ISBNs
978-1-4939-2729-6, 978-1-4939-2730-2
Authors

Shibata, Tomonori, Suzuki, Yuki, Sugiyama, Hiroshi, Endo, Masayuki, Saito, Hirohide, Tomonori Shibata, Yuki Suzuki, Hiroshi Sugiyama, Masayuki Endo, Hirohide Saito

Abstract

RNA-protein (RNP) complexes are promising biomaterials for the fields of nanotechnology and synthetic biology. Protein-responsive RNA sequences (RNP motifs) can be integrated into various RNAs, such as messenger RNA, short-hairpin RNA, and synthetic RNA nano-objects for a variety of purposes. Direct observation of RNP interaction in solution at high resolution is important in the design and construction of RNP-mediated nanostructures. Here we describe a method to construct and visualize RNP nanostructures that precisely arrange a target protein on the RNA scaffold with nanometer scale. High-speed AFM (HS-AFM) images of RNP nanostructures show that the folding of RNP complexes of defined sizes can be directly visualized at single RNP resolution in solution.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Professor 3 50%
Researcher 2 33%
Unknown 1 17%
Readers by discipline Count As %
Chemistry 3 50%
Biochemistry, Genetics and Molecular Biology 2 33%
Unknown 1 17%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 May 2015.
All research outputs
#20,273,512
of 22,805,349 outputs
Outputs from Methods in molecular biology
#9,905
of 13,120 outputs
Outputs of similar age
#295,802
of 353,075 outputs
Outputs of similar age from Methods in molecular biology
#635
of 996 outputs
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