| Title |
A three-step method for analysing bacterial biofilm formation under continuous medium flow
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, May 2015
|
| DOI | 10.1007/s00253-015-6628-8 |
| Pubmed ID | |
| Authors |
Karolin Schmutzler, Andreas Schmid, Katja Buehler |
| Abstract |
For the investigation and comparison of microbial biofilms, a variety of analytical methods have been established, all focusing on different growth stages and application areas of biofilms. In this study, a novel quantitative assay for analysing biofilm maturation under the influence of continuous flow conditions was developed using the interesting biocatalyst Pseudomonas taiwanensis VLB120. In contrast to other tubular-based assay systems, this novel assay format delivers three readouts using a single setup in a total assay time of 40 h. It combines morphotype analysis of biofilm colonies with the direct quantification of biofilm biomass and pellicle formation on an air/liquid interphase. Applying the Tube-Assay, the impact of the second messenger cyclic diguanylate on biofilm formation of P. taiwanensis VLB120 was investigated. To this end, 41 deletions of genes encoding for protein homologues to diguanylate cyclase and phosphodiesterase were generated in the genome of P. taiwanensis VLB120. Subsequently, the biofilm formation of the resulting mutants was analysed using the Tube-Assay. In more than 60 % of the mutants, a significantly altered biofilm formation as compared to the parent strain was detected. Furthermore, the potential of the proposed Tube-Assay was validated by investigating the biofilms of several other bacterial species. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 3% |
| Brazil | 1 | 3% |
| Unknown | 30 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 25% |
| Student > Doctoral Student | 4 | 13% |
| Student > Postgraduate | 3 | 9% |
| Student > Master | 3 | 9% |
| Student > Bachelor | 2 | 6% |
| Other | 7 | 22% |
| Unknown | 5 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 9 | 28% |
| Biochemistry, Genetics and Molecular Biology | 7 | 22% |
| Environmental Science | 2 | 6% |
| Unspecified | 2 | 6% |
| Business, Management and Accounting | 1 | 3% |
| Other | 3 | 9% |
| Unknown | 8 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 March 2016.
All research outputs
#16,371,088
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#5,817
of 8,034 outputs
Outputs of similar age
#161,124
of 268,442 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#62
of 124 outputs
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