| Title |
PMT1 deficiency enhances basal UPR activity and extends replicative lifespan of Saccharomyces cerevisiae
|
|---|---|
| Published in |
GeroScience, May 2015
|
| DOI | 10.1007/s11357-015-9788-7 |
| Pubmed ID | |
| Authors |
Hong-Jing Cui, Xin-Guang Liu, Mark McCormick, Brian M. Wasko, Wei Zhao, Xin He, Yuan Yuan, Bing-Xiong Fang, Xue-Rong Sun, Brian K. Kennedy, Yousin Suh, Zhong-Jun Zhou, Matt Kaeberlein, Wen-Li Feng |
| Abstract |
Pmt1p is an important member of the protein O-mannosyltransferase (PMT) family of enzymes, which participates in the endoplasmic reticulum (ER) unfolded protein response (UPR), an important pathway for alleviating ER stress. ER stress and the UPR have been implicated in aging and age-related diseases in several organisms; however, a possible role for PMT1 in determining lifespan has not been previously described. In this study, we report that deletion of PMT1 increases replicative lifespan (RLS) in the budding yeast Saccharomyces cerevisiae, while overexpression of PMT1 (PMT1-OX) reduces RLS. Relative to wild-type and PMT1-OX strains, the pmt1Δ strain had enhanced HAC1 mRNA splicing and elevated expression levels of UPR target genes. Furthermore, the increased RLS of the pmt1Δ strain could be completely abolished by deletion of either IRE1 or HAC1, two upstream modulators of the UPR. The double deletion strains pmt1Δhac1Δ and pmt1Δire1Δ also displayed generally reduced transcription of UPR target genes. Collectively, our results suggest that PMT1 deficiency enhances basal activity of the ER UPR and extends the RLS of yeast mother cells through a mechanism that requires both IRE1 and HAC1. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 31 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 13% |
| Other | 3 | 10% |
| Student > Bachelor | 3 | 10% |
| Researcher | 3 | 10% |
| Student > Master | 3 | 10% |
| Other | 4 | 13% |
| Unknown | 11 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 23% |
| Engineering | 4 | 13% |
| Agricultural and Biological Sciences | 2 | 6% |
| Arts and Humanities | 1 | 3% |
| Linguistics | 1 | 3% |
| Other | 6 | 19% |
| Unknown | 10 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 May 2015.
All research outputs
#22,759,452
of 25,373,627 outputs
Outputs from GeroScience
#1,512
of 1,594 outputs
Outputs of similar age
#239,407
of 279,058 outputs
Outputs of similar age from GeroScience
#23
of 28 outputs
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