| Title |
Transdifferentiation of Bone Marrow Mesenchymal Stem Cells into the Islet-Like Cells: the Role of Extracellular Matrix Proteins
|
|---|---|
| Published in |
Archivum Immunologiae et Therapiae Experimentalis, May 2015
|
| DOI | 10.1007/s00005-015-0340-3 |
| Pubmed ID | |
| Authors |
Marta Pokrywczynska, Marzena Anna Lewandowska, Sandra Krzyzanowska, Arkadiusz Jundzill, Marta Rasmus, Karolina Warda, Maciej Gagat, Aleksander Deptula, Anna Helmin-Basa, Marcin Holysz, Maciej Nowacki, Lukasz Buchholz, Magdalena Bodnar, Andrzej Marszalek, Alina Grzanka, Wojciech Jozwicki, Jacek Michalkiewicz, Tomasz Drewa |
| Abstract |
Pancreatic islet implantation has been recently shown to be an efficient method of treatment for type 1 diabetes. However, limited availability of donor islets reduces its use. Bone morrow would provide potentially unlimited source of stem cells for generation of insulin-producing cells. This study was performed to evaluate the influence of extracellular matrix proteins like collagen, laminin, and vitronectin on bone marrow mesenchymal stem cells (BM-MSCs) transdifferentiation into islet-like cells (ILCs) in vitro. To our knowledge, this is the first report evaluating the importance of vitronectin in transdifferentiation of BM-MSCs into ILCs. Rat BM-MSCs were induced to ILCs using four-step protocol on plates coated with collagen type IV, laminin type I and vitronectin type I. Quantitative real-time PCR was performed to detect gene expression related to pancreatic β cell development. The induced cells expressed islet-related genes including: neurogenin 3, neurogenic differentiation 1, paired box 4, NK homeobox factor 6.1, glucagon, insulin 1 and insulin 2. Laminin but not collagen type IV or vitronectin enhanced expression of insulin and promoted formation of islet-like structures in monolayer culture. Laminin triggered transdifferentiation of BM-MSCs into ILCs. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 3% |
| Unknown | 34 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 23% |
| Student > Bachelor | 7 | 20% |
| Student > Master | 5 | 14% |
| Researcher | 3 | 9% |
| Professor | 2 | 6% |
| Other | 7 | 20% |
| Unknown | 3 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 9 | 26% |
| Biochemistry, Genetics and Molecular Biology | 8 | 23% |
| Agricultural and Biological Sciences | 7 | 20% |
| Immunology and Microbiology | 2 | 6% |
| Engineering | 2 | 6% |
| Other | 2 | 6% |
| Unknown | 5 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 May 2015.
All research outputs
#18,410,971
of 22,805,349 outputs
Outputs from Archivum Immunologiae et Therapiae Experimentalis
#281
of 385 outputs
Outputs of similar age
#191,901
of 263,961 outputs
Outputs of similar age from Archivum Immunologiae et Therapiae Experimentalis
#4
of 6 outputs
Altmetric has tracked 22,805,349 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 385 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,961 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 6 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.