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Hypoxia limits mouse follicle growth in vitro

Overview of attention for article published in Reproduction, Fertility, & Development, April 2015
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Title
Hypoxia limits mouse follicle growth in vitro
Published in
Reproduction, Fertility, & Development, April 2015
DOI 10.1071/rd14471
Pubmed ID
Authors

J. M. Connolly, M. T. Kane, L. R. Quinlan, P. Dockery, A. C. Hynes

Abstract

Ovarian follicle culture is useful for elucidation of factors involved in the regulation of follicular function. We examined the effects of gas phase oxygen concentration, an oil overlay, serum type and medium supplementation with FSH, insulin-transferrin-selenium (ITS) and l-ascorbic acid on cultured preantral mouse follicle growth in a spherical, non-attached follicle culture system. Follicle growth in 5% oxygen was significantly (PPP>0.05) affected by an oil overlay, ITS supplementation or serum type. Culture in medium with 5% mouse serum, 1IUmL-1 FSH, 25?gmL-1 l-ascorbic acid and 20% oxygen without an oil overlay supported the growth of follicles to a maximum diameter of 380?m in 6 days. Compared with mature preovulatory mouse follicles in vivo that often have diameters >500?m within the same time frame, in vitro-grown follicles clearly exhibit limited growth. Thus, adequate oxygenation is an essential factor in the process of optimising follicle growth.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 2 18%
Professor 1 9%
Student > Bachelor 1 9%
Student > Ph. D. Student 1 9%
Unknown 6 55%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 9%
Materials Science 1 9%
Chemistry 1 9%
Medicine and Dentistry 1 9%
Unknown 7 64%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 April 2015.
All research outputs
#22,793,536
of 25,411,814 outputs
Outputs from Reproduction, Fertility, & Development
#919
of 1,179 outputs
Outputs of similar age
#240,295
of 279,219 outputs
Outputs of similar age from Reproduction, Fertility, & Development
#20
of 32 outputs
Altmetric has tracked 25,411,814 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,179 research outputs from this source. They receive a mean Attention Score of 3.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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