| Title |
Enhanced production of porcine circovirus type 2 (PCV2) virus-like particles in Sf9 cells by translational enhancers
|
|---|---|
| Published in |
Biotechnology Techniques, May 2015
|
| DOI | 10.1007/s10529-015-1856-7 |
| Pubmed ID | |
| Authors |
Yangkun Liu, Yuanyuan Zhang, Lunguang Yao, Huafang Hao, Xiangjing Fu, Zengqi Yang, Enqi Du |
| Abstract |
To investigate the effect of three translational enhancers for enhancing transgene expression in baculovirus expression vector system using GFP as a reporter gene and selected translational enhancers to increase porcine circovirus type 2 (PCV2) VLPs production. P10UTR (the 3'-untranslated region from the baculovirus p10 gene), Syn21 (a synthetic AT-rich 21-bp sequence) and P10UTR/Syn21 increased the GFP yield by 1.4-, 4- and 4.8-fold, respectively. While IVS (intron from Drosophila myosin heavy chain gene) decreased the GFP yield by 65 %. Moreover, the synergy of P10UTR/Syn21 increased the yield of PCV2 VLPs by 4.1 fold (45 μg/10(6) cells) compared with standard baculovirus vector. The synergy of P10UTR/Syn21 is a potential strategy to improve the recombinant vaccine production besides PCV2 VLPs in BEVS. |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 18% |
| Student > Ph. D. Student | 3 | 18% |
| Student > Bachelor | 2 | 12% |
| Student > Master | 2 | 12% |
| Student > Doctoral Student | 1 | 6% |
| Other | 2 | 12% |
| Unknown | 4 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 29% |
| Agricultural and Biological Sciences | 4 | 24% |
| Veterinary Science and Veterinary Medicine | 2 | 12% |
| Earth and Planetary Sciences | 1 | 6% |
| Medicine and Dentistry | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 4 | 24% |