Keratinocytes play a pivotal role in the regulation of immune responses but the impact of antigen-presentation by these cells is still poorly understood, particularly in a situation where the antigen will be presented only in adult life. Here, we generated a transgenic mouse model in which keratinocytes exclusively present a myelin basic protein (MBP) peptide covalently linked to the MHC class II β-chain, solely under inflammatory conditions. In these mice, inflammation caused by epicutaneous contact sensitizer treatment resulted in keratinocyte-mediated expansion of MBP-specific CD4(+) T cells in the skin. Moreover, repeated contact sensitizer application preceding a systemic MBP immunization reduced the reactivity of the respective CD4(+) T cells and lowered the symptoms of the resulting experimental autoimmune encephalomyelitis. This down-regulation was CD4(+) T cell mediated and dependent on the presence of the immune modulator Dickkopf-3. Thus, presentation of a neo self-antigen by keratinocytes in the inflamed, adult skin can modulate CD4(+) T cell auto-aggression at a distal organ.In parallel to the DPCP skin treatments, 0.5 mg of the anti-Dkk3 4.22 mAb (Papatriantafyllou et al., 2012) in 200 μl PBS was applied i.p., 3 times, each one week apart.Journal of Investigative Dermatology accepted article preview online, 02 April 2015. doi:10.1038/jid.2015.130.
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 November 2015.
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Outputs of similar age from Journal of Investigative Dermatology
#43
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Altmetric has tracked 25,371,288 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,994 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 8.4. This one has done well, scoring higher than 87% of its peers.
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