Title |
Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses
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Published in |
Virology, May 2015
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DOI | 10.1016/j.virol.2015.05.001 |
Pubmed ID | |
Authors |
Nicolás Bejerman, Fabián Giolitti, Soledad de Breuil, Verónica Trucco, Claudia Nome, Sergio Lenardon, Ralf G. Dietzgen |
Abstract |
We have determined the full-length 14,491-nucleotide genome sequence of a new plant rhabdovirus, alfalfa dwarf virus (ADV). Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3'-N-P-P3-M-G-P6-L-5'. The ORFs are separated by conserved intergenic regions and the genome coding region is flanked by complementary 3' leader and 5' trailer sequences. Phylogenetic analysis of the nucleoprotein amino acid sequence indicated that this alfalfa-infecting rhabdovirus is related to viruses in the genus Cytorhabdovirus. When transiently expressed as GFP fusions in Nicotiana benthamiana leaves, most ADV proteins accumulated in the cell periphery, but unexpectedly P protein was localized exclusively in the nucleus. ADV P protein was shown to have a homotypic, and heterotypic nuclear interactions with N, P3 and M proteins by bimolecular fluorescence complementation. ADV appears unique in that it combines properties of both cytoplasmic and nuclear plant rhabdoviruses. |
X Demographics
Geographical breakdown
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Unknown | 2 | 100% |
Demographic breakdown
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
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Indonesia | 1 | 3% |
Argentina | 1 | 3% |
Unknown | 27 | 93% |
Demographic breakdown
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Researcher | 7 | 24% |
Student > Postgraduate | 5 | 17% |
Student > Master | 4 | 14% |
Student > Doctoral Student | 3 | 10% |
Student > Bachelor | 3 | 10% |
Other | 6 | 21% |
Unknown | 1 | 3% |
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Computer Science | 1 | 3% |
Nursing and Health Professions | 1 | 3% |
Other | 2 | 7% |
Unknown | 2 | 7% |