Title |
H2O2-induced secretion of tumor necrosis factor-α evokes apoptosis of cardiac myocytes through reactive oxygen species-dependent activation of p38 MAPK
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Published in |
Methods in Cell Science, October 2011
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DOI | 10.1007/s10616-011-9392-3 |
Pubmed ID | |
Authors |
Zhilong Chen, Hong Jiang, Yanwu Wan, Chaofang Bi, Yian Yuan |
Abstract |
P38 mitogen-activated protein kinases (p38 MAPK) and tumor necrosis factor-α (TNF-α) play important roles in oxidative stress-induced apoptosis in cardiac myocytes. However, the regulation and functional role of cross-talk between p38 MAPK and TNF-α pathways have not yet been fully characterized in cardiac myocytes. In this study, we found that inhibition of p38 MAPK with SB-203580 (SB) reduced H(2)O(2)-stimulated secretion of TNF-α, whereas pre-activation of p38 MAPK with sodium arsenite (SA) enhanced H(2)O(2)-stimulated secretion of TNF-α. In addition, pretreatment of cells with TNF-α increased basal and H(2)O(2)-stimulated p38 MAPK and apoptosis of cardiac myocytes, and p38 MAPK-associated apoptosis of cardiac myocytes induced by TNF-α was blocked by inhibition of p38 MAPK with SB. Finally, H(2)O(2)-induced apoptosis was attenuated by the inhibitors of p38 MAPK or reactive oxygen species (ROS), whereas it was enhanced by p38 MAPK agonist SA. These results suggest that H(2)O(2)-induced secretion of TNF-α increases apoptosis of cardiac myocytes through ROS-dependent activation of p38 MAPK. This may represent a novel mechanism that TNF-α partly interplays with p38 MAPK pathways during oxidative stress-modulated apoptosis in cardiac myocytes. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Postgraduate | 3 | 27% |
Student > Ph. D. Student | 3 | 27% |
Student > Doctoral Student | 2 | 18% |
Researcher | 1 | 9% |
Student > Master | 1 | 9% |
Other | 0 | 0% |
Unknown | 1 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 4 | 36% |
Agricultural and Biological Sciences | 3 | 27% |
Veterinary Science and Veterinary Medicine | 1 | 9% |
Unknown | 3 | 27% |