| Title |
Metabolomic analysis of wild and transgenic Nicotiana langsdorffii plants exposed to abiotic stresses: unraveling metabolic responses
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, May 2015
|
| DOI | 10.1007/s00216-015-8770-7 |
| Pubmed ID | |
| Authors |
Elisa Scalabrin, Marta Radaelli, Giovanni Rizzato, Patrizia Bogani, Marcello Buiatti, Andrea Gambaro, Gabriele Capodaglio |
| Abstract |
Nicotiana langsdorffii plants, wild and transgenic for the Agrobacterium rhizogenes rol C gene and the rat glucocorticoid receptor (GR) gene, were exposed to different abiotic stresses (high temperature, water deficit, and high chromium concentrations). An untargeted metabolomic analysis was carried out in order to investigate the metabolic effects of the inserted genes in response to the applied stresses and to obtain a comprehensive profiling of metabolites induced during abiotic stresses. High-performance liquid chromatography separation (HPLC) coupled to high-resolution mass spectrometry (HRMS) enabled the identification of more than 200 metabolites, and statistical analysis highlighted the most relevant compounds for each plant treatment. The plants exposed to heat stress showed a unique set of induced secondary metabolites, some of which were known while others were not previously reported for this kind of stress; significant changes were observed especially in lipid composition. The role of trichome, as a protection against heat stress, is here suggested by the induction of both acylsugars and glykoalkaloids. Water deficit and Cr(VI) stresses resulted mainly in enhanced antioxidant (HCAs, polyamine) levels and in the damage of lipids, probably as a consequence of reactive oxygen species (ROS) production. Moreover, the ability of rol C expression to prevent oxidative burst was confirmed. The results highlighted a clear influence of GR modification on plant stress response, especially to water deficiency-a phenomenon whose applications should be further investigated. This study provides new insights into the field of system biology and demonstrates the importance of metabolomics in the study of plant functioning. Graphical Abstract Untargeted metabolomic analysis was applied to wild type, GR and RolC modified Nicotiana Langsdorffii plants exposed to heat, water and Cr(VI) stresses. The key metabolites, highly affected by stress application, were identified, allowing to outline the main metabolic responses to stress in each plant genotype. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 1 | 2% |
| Netherlands | 1 | 2% |
| China | 1 | 2% |
| Brazil | 1 | 2% |
| Unknown | 57 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 28% |
| Researcher | 9 | 15% |
| Student > Master | 6 | 10% |
| Professor | 5 | 8% |
| Student > Postgraduate | 4 | 7% |
| Other | 10 | 16% |
| Unknown | 10 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 25 | 41% |
| Chemistry | 6 | 10% |
| Biochemistry, Genetics and Molecular Biology | 5 | 8% |
| Environmental Science | 3 | 5% |
| Medicine and Dentistry | 2 | 3% |
| Other | 5 | 8% |
| Unknown | 15 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 August 2015.
All research outputs
#19,945,185
of 25,374,917 outputs
Outputs from Analytical & Bioanalytical Chemistry
#6,060
of 9,619 outputs
Outputs of similar age
#192,202
of 280,167 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#63
of 197 outputs
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