| Title |
miR-375 gene dosage in pancreatic β-cells: implications for regulation of β-cell mass and biomarker development
|
|---|---|
| Published in |
Journal of Molecular Medicine, May 2015
|
| DOI | 10.1007/s00109-015-1296-9 |
| Pubmed ID | |
| Authors |
Mathieu Latreille, Karolin Herrmanns, Neil Renwick, Thomas Tuschl, Maciej T. Malecki, Mark I. McCarthy, Katharine R. Owen, Thomas Rülicke, Markus Stoffel |
| Abstract |
MicroRNAs play a crucial role in the regulation of cell growth and differentiation. Mice with genetic deletion of miR-375 exhibit impaired glycemic control due to decreased β-cell and increased α-cell mass and function. The relative importance of these processes for the overall phenotype of miR-375KO mice is unknown. Here, we show that mice overexpressing miR-375 exhibit normal β-cell mass and function. Selective re-expression of miR-375 in β-cells of miR-375KO mice normalizes both, α- and β-cell phenotypes as well as glucose metabolism. Using this model, we also analyzed the contribution of β-cells to the total plasma miR-375 levels. Only a small proportion (≈1 %) of circulating miR-375 originates from β-cells. Furthermore, acute and profound β-cell destruction is sufficient to detect elevations of miR-375 levels in the blood. These findings are supported by higher miR-375 levels in the circulation of type 1 diabetes (T1D) subjects but not mature onset diabetes of the young (MODY) and type 2 diabetes (T2D) patients. Together, our data support an essential role for miR-375 in the maintenance of β-cell mass and provide in vivo evidence for release of miRNAs from pancreatic β-cells. The small contribution of β-cells to total plasma miR-375 levels make this miRNA an unlikely biomarker for β-cell function but suggests a utility for the detection of acute β-cell death for autoimmune diabetes. Overexpression of miR-375 in β-cells does not influence β-cell mass and function. Increased α-cell mass in miR-375KO arises secondarily to loss of miR-375 in β-cells. Only a small proportion of circulating miR-375 levels originates from β-cells. Acute β-cell destruction results in measurable increases of miR-375 in the blood. Circulating miR-375 levels are not a biomarker for pancreatic β-cell function. |
X Demographics
The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Costa Rica | 1 | 14% |
| United States | 1 | 14% |
| Unknown | 5 | 71% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 6 | 86% |
| Scientists | 1 | 14% |
Mendeley readers
The data shown below were compiled from readership statistics for 109 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 109 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 26 | 24% |
| Researcher | 17 | 16% |
| Student > Master | 17 | 16% |
| Student > Bachelor | 7 | 6% |
| Student > Doctoral Student | 5 | 5% |
| Other | 11 | 10% |
| Unknown | 26 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 31 | 28% |
| Agricultural and Biological Sciences | 16 | 15% |
| Medicine and Dentistry | 13 | 12% |
| Pharmacology, Toxicology and Pharmaceutical Science | 6 | 6% |
| Nursing and Health Professions | 3 | 3% |
| Other | 9 | 8% |
| Unknown | 31 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 25. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 August 2022.
All research outputs
#1,432,261
of 24,203,404 outputs
Outputs from Journal of Molecular Medicine
#35
of 1,596 outputs
Outputs of similar age
#18,353
of 270,700 outputs
Outputs of similar age from Journal of Molecular Medicine
#3
of 22 outputs
Altmetric has tracked 24,203,404 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 94th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,596 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.4. This one has done particularly well, scoring higher than 97% of its peers.
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We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 90% of its contemporaries.