| Title |
Nanosized aptameric cavities imprinted on the surface of magnetic nanoparticles for high-throughput protein recognition
|
|---|---|
| Published in |
Microchimica Acta, March 2018
|
| DOI | 10.1007/s00604-018-2745-2 |
| Pubmed ID | |
| Authors |
Elham Shoghi, Seyede Zohreh Mirahmadi-Zare, Razieh Ghasemi, Matine Asghari, Mansour Poorebrahim, Mohammad-Hossein Nasr-Esfahani |
| Abstract |
The authors introduce a new kind of surface artificial biomimetic receptor, referred to as aptameric imprinted polymer (AIP), for separation of biological macromolecules. Highly dispersed magnetic nanoparticles (MNPs) were coated with silica and then functionalized with methacrylate groups via silane chemistry. The aptamer was covalently immobilized on the surface of nanoparticles via a "thiol-ene" click reaction. Once the target analyte (bovine serum albumin; BSA) has bound to the aptamer, a polymer is created by 2-dimensional copolymerization of short-length poly(ethylene glycol) and (3-aminopropyl)triethoxysilane. Following removal of BSA from the polymer, the AIP-MNPs presented here can selectively capture BSA with a specific absorbance (κ) as high as 65. When using this AIP, the recovery of BSA from spiked real biological samples is >97%, and the adsorption capacity is as high as 146 mg g-1. In our perception, this method has a wide scope in that it may be applied to the specific extraction of numerous other biomolecules. Graphical abstract Schematic presentation of the AIP (aptamer-imprinted polymer) introduced here. The surface of silica coated magnetic nanoparticles is modified with a polymer that is covalently modified with an aptamer against bovine serum albumin (BSA). |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 11% |
| Student > Master | 2 | 11% |
| Student > Doctoral Student | 1 | 5% |
| Student > Bachelor | 1 | 5% |
| Other | 1 | 5% |
| Other | 3 | 16% |
| Unknown | 9 | 47% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 4 | 21% |
| Chemical Engineering | 1 | 5% |
| Neuroscience | 1 | 5% |
| Biochemistry, Genetics and Molecular Biology | 1 | 5% |
| Materials Science | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 10 | 53% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 April 2018.
All research outputs
#20,485,225
of 23,047,237 outputs
Outputs from Microchimica Acta
#1,039
of 1,401 outputs
Outputs of similar age
#291,400
of 330,032 outputs
Outputs of similar age from Microchimica Acta
#15
of 31 outputs
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