| Title |
Aptamer based vanillin sensor using an ion-sensitive field-effect transistor
|
|---|---|
| Published in |
Microchimica Acta, December 2017
|
| DOI | 10.1007/s00604-017-2586-4 |
| Pubmed ID | |
| Authors |
Alexander Kuznetsov, Natalia Komarova, Maria Andrianova, Vitaliy Grudtsov, Evgeniy Kuznetsov |
| Abstract |
An aptamer for vanillin was obtained and then used for the development of an aptasensor based on an ion-sensitive field-effect transistor (ISFET). This aptamer (a single-stranded DNA;ssDNA) was selected using the Capture-SELEX protocol, which suites well for selection of aptamers to small molecules. Among six aptamer candidates, the aptamer Van_74 with the highest affinity for vanillin was chosen (elution of 35% of the aptamer from a solid support in the presence of 2 mM of vanillin). Van_74 was characterized using nondenaturating PAGE of washouts from magnetic beads. It is shown that Van_74 binds to vanillin with an dissociation constant of >7.8 μM (determined by nondenaturating PAGE) and it was specific to vanillin in comparison with interferents: benzaldehyde, guaiacol, furaneol, ethyl guaiacol and ethyl vanillin. Also it was shown that change of buffer composition greatly affected the binding ability of Van_74. For biosensor fabrication aptamer was immobilised on the Ta2O5-sensitive surface of the ISFET via "click-chemistry". Detection scheme implied dehybridisation of the ssDNA probe from the aptamer and release in the solution during the addition of vanillin. As a result, the surface potential increase upon vanillin binding with the aptamer was detected by the transistor. The biosensor had a detection limit of 1.55 × 10-7 M and a dynamic range from 1.55 × 10-7 M to 1 × 10-6 M. Effective constant Kd,eff for vanillin binding on biosensor surface was calculated to be (9 ± 3) × 10-7 M. This allows selective detection of vanillin in the mixture of interferents and in samples of coffee extract. Graphical abstract A biosensor for vanillin was developed on the basis of an aptamer that was obtained via Capture-SELEX and by using an ISFET. This biosensor can be used for vanillin detection in presence of interferents and in real sample using an approach of ssDNA probe dehybridization. |
Mendeley readers
The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 36 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 22% |
| Researcher | 8 | 22% |
| Student > Doctoral Student | 5 | 14% |
| Student > Bachelor | 2 | 6% |
| Student > Master | 2 | 6% |
| Other | 2 | 6% |
| Unknown | 9 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 10 | 28% |
| Biochemistry, Genetics and Molecular Biology | 7 | 19% |
| Engineering | 4 | 11% |
| Physics and Astronomy | 1 | 3% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 13 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2024.
All research outputs
#4,664,782
of 23,047,237 outputs
Outputs from Microchimica Acta
#72
of 1,401 outputs
Outputs of similar age
#99,840
of 438,271 outputs
Outputs of similar age from Microchimica Acta
#3
of 29 outputs
Altmetric has tracked 23,047,237 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,401 research outputs from this source. They receive a mean Attention Score of 2.4. This one has done particularly well, scoring higher than 94% of its peers.
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We're also able to compare this research output to 29 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.