Title |
Distinct Spacing Between Anionic Groups: An Essential Chemical Determinant for Achieving Thiophene‐Based Ligands to Distinguish β‐Amyloid or Tau Polymorphic Aggregates
|
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Published in |
Chemistry - A European Journal, May 2015
|
DOI | 10.1002/chem.201500556 |
Pubmed ID | |
Authors |
Therése Klingstedt, Hamid Shirani, Jasmin Mahler, Bettina M Wegenast-Braun, Sofie Nyström, Michel Goedert, Mathias Jucker, K Peter R Nilsson |
Abstract |
The accumulation of protein aggregates is associated with many devastating neurodegenerative diseases and the existence of distinct aggregated morphotypes has been suggested to explain the heterogeneous phenotype reported for these diseases. Thus, the development of molecular probes able to distinguish such morphotypes is essential. We report an anionic tetrameric oligothiophene compound that can be utilized for spectral assignment of different morphotypes of β-amyloid or tau aggregates present in transgenic mice at distinct ages. The ability of the ligand to spectrally distinguish between the aggregated morphotypes was reduced when the spacing between the anionic substituents along the conjugated thiophene backbone was altered, which verified that specific molecular interactions between the ligand and the protein aggregate are necessary to detect aggregate polymorphism. Our findings provide the structural and functional basis for the development of new fluorescent ligands that can distinguish between different morphotypes of protein aggregates. |
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Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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United States | 1 | 3% |
France | 1 | 3% |
Germany | 1 | 3% |
Unknown | 29 | 91% |
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Student > Ph. D. Student | 8 | 25% |
Other | 4 | 13% |
Researcher | 4 | 13% |
Student > Bachelor | 3 | 9% |
Lecturer > Senior Lecturer | 2 | 6% |
Other | 4 | 13% |
Unknown | 7 | 22% |
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Immunology and Microbiology | 1 | 3% |
Other | 1 | 3% |
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