| Title |
The influence of oxygen and methane on nitrogen fixation in subarctic Sphagnum mosses
|
|---|---|
| Published in |
AMB Express, May 2018
|
| DOI | 10.1186/s13568-018-0607-2 |
| Pubmed ID | |
| Authors |
Martine A. R. Kox, Sanni L. Aalto, Timo Penttilä, Katharina F. Ettwig, Mike S. M. Jetten, Maartje A. H. J. van Kessel |
| Abstract |
Biological nitrogen fixation is an important source of bioavailable nitrogen in Sphagnum dominated peatlands. Sphagnum mosses harbor a diverse microbiome including nitrogen-fixing and methane (CH4) oxidizing bacteria. The inhibitory effect of oxygen on microbial nitrogen fixation is documented for many bacteria. However, the role of nitrogen-fixing methanotrophs in nitrogen supply to Sphagnum peat mosses is not well explored. Here, we investigated the role of both oxygen and methane on nitrogen fixation in subarctic Sphagnum peat mosses. Five species of Sphagnum mosses were sampled from two mesotrophic and three oligotrophic sites within the Lakkasuo peatland in Orivesi, central Finland. Mosses were incubated under either ambient or low oxygen conditions in the presence or absence of methane. Stable isotope activity assays revealed considerable nitrogen-fixing and methane-assimilating rates at all sites (1.4 ± 0.2 µmol 15N-N2 g-1 DW day-1 and 12.0 ± 1.1 µmol 13C-CH4 g-1 DW day-1, respectively). Addition of methane did not stimulate incorporation of 15N-nitrogen into biomass, whereas oxygen depletion increased the activity of the nitrogen-fixing community. Analysis of the 16S rRNA genes at the bacterial community level showed a very diverse microbiome that was dominated by Alphaproteobacteria in all sites. Bona fide methane-oxidizing taxa were not very abundant (relative abundance less than 0.1%). Based on our results we conclude that methanotrophs did not contribute significantly to nitrogen fixation in the investigated peatlands. |
X Demographics
The data shown below were collected from the profiles of 12 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Netherlands | 5 | 42% |
| United Kingdom | 1 | 8% |
| Sweden | 1 | 8% |
| Unknown | 5 | 42% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 7 | 58% |
| Members of the public | 5 | 42% |
Mendeley readers
The data shown below were compiled from readership statistics for 55 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 55 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 15 | 27% |
| Student > Ph. D. Student | 5 | 9% |
| Student > Bachelor | 5 | 9% |
| Student > Master | 5 | 9% |
| Student > Doctoral Student | 2 | 4% |
| Other | 6 | 11% |
| Unknown | 17 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 22% |
| Environmental Science | 10 | 18% |
| Biochemistry, Genetics and Molecular Biology | 6 | 11% |
| Chemistry | 4 | 7% |
| Immunology and Microbiology | 2 | 4% |
| Other | 4 | 7% |
| Unknown | 17 | 31% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 January 2019.
All research outputs
#6,490,914
of 24,597,084 outputs
Outputs from AMB Express
#117
of 1,295 outputs
Outputs of similar age
#105,768
of 332,214 outputs
Outputs of similar age from AMB Express
#7
of 59 outputs
Altmetric has tracked 24,597,084 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 1,295 research outputs from this source. They receive a mean Attention Score of 2.9. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 332,214 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 59 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 89% of its contemporaries.