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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Nuclear localized Akt enhances breast cancer stem-like cells through counter-regulation of p21Waf1/Cip1 and p27kip1
|
|---|---|
| Published in |
Cell Cycle, June 2015
|
| DOI | 10.1080/15384101.2015.1041692 |
| Pubmed ID | |
| Authors |
Mayur Vilas Jain, Jaganmohan R Jangamreddy, Jerzy Grabarek, Frank Schweizer, Thomas Klonisch, Artur Cieślar-Pobuda, Marek J Łos |
| Abstract |
Cancer stem-like cells (CSCs) are a rare subpopulation of cancer cells capable of propagating the disease and causing cancer recurrence. In this study, we found that the cellular localization of PKB/Akt kinase affects the maintenance of CSCs. When Akt tagged with nuclear localization signal (Akt-NLS) was overexpressed in SKBR3 and MDAMB468 cells, these cells showed a 10-15% increase in the number of cells with CSCs enhanced ALDH activity and showing CD44(+High)/CD24(-Low) phenotype. This effect was completely reversed in the presence of Akt-specific inhibitor, Triciribine. Furthermore, cells overexpressing Akt or Akt-NLS were less likely to be in G0/G1 phase of the cell cycle by inactivating p21(Waf1/Cip1) and exhibited increased clonogenicity and proliferation as assayed by colony-forming assay (mammosphere formation). Thus, our data emphasize the importance the intracellular localization of Akt has on stemness in human breast cancer cells. It also indicates a new robust way for improving the enrichment and culture of CSCs for experimental purposes. Hence, it allows for the development of simpler protocols to study stemness, clonogenic potency, and screening of new chemotherapeutic agents that preferentially target cancer stem cells. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 40 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 40 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 23% |
| Student > Bachelor | 5 | 13% |
| Student > Master | 4 | 10% |
| Student > Doctoral Student | 3 | 8% |
| Researcher | 3 | 8% |
| Other | 8 | 20% |
| Unknown | 8 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 13 | 33% |
| Pharmacology, Toxicology and Pharmaceutical Science | 5 | 13% |
| Medicine and Dentistry | 5 | 13% |
| Agricultural and Biological Sciences | 4 | 10% |
| Immunology and Microbiology | 1 | 3% |
| Other | 3 | 8% |
| Unknown | 9 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 May 2020.
All research outputs
#6,955,162
of 22,808,725 outputs
Outputs from Cell Cycle
#730
of 3,684 outputs
Outputs of similar age
#81,239
of 264,367 outputs
Outputs of similar age from Cell Cycle
#19
of 101 outputs
Altmetric has tracked 22,808,725 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 3,684 research outputs from this source. They receive a mean Attention Score of 3.7. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,367 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.
We're also able to compare this research output to 101 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 81% of its contemporaries.