| Title |
High-Fat Diet Consumption Induces Microbiota Dysbiosis and Intestinal Inflammation in Zebrafish
|
|---|---|
| Published in |
Microbial Ecology, May 2018
|
| DOI | 10.1007/s00248-018-1198-9 |
| Pubmed ID | |
| Authors |
Nerea Arias-Jayo, Leticia Abecia, Laura Alonso-Sáez, Andoni Ramirez-Garcia, Alfonso Rodriguez, Miguel A. Pardo |
| Abstract |
Energy-dense foods and overnutrition represent major starting points altering lipid metabolism, systemic inflammation and gut microbiota. The aim of this work was to investigate the effects of a high-fat diet (HFD) over a period of 25 days on intestinal microbiota and inflammation in zebrafish. Microbial composition of HFD-fed animals was analysed and compared to controls by 16S rRNA sequencing and quantitative PCR. The expression level on several genes related to inflammation was tested. Furthermore, microscopic assessment of the intestine was performed in both conditions. The consumption of the HFD resulted in microbial dysbiosis, characterised by an increase in the relative abundance of the phylum Bacteroidetes. Moreover, an emerging intestinal inflammation via NF-κβ activation was confirmed by the overexpression of several genes related to signalling receptors, antimicrobial metabolism and the inflammatory cascade. The intestinal barrier was also damaged, with an increase of goblet cell mucin production. This is the first study performed in zebrafish which suggests that the consumption of a diet enriched with 10% fat changes the intestinal microbial community composition, which was correlated with low-grade inflammation. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 120 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 120 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 22 | 18% |
| Researcher | 16 | 13% |
| Student > Ph. D. Student | 14 | 12% |
| Student > Master | 11 | 9% |
| Professor > Associate Professor | 6 | 5% |
| Other | 16 | 13% |
| Unknown | 35 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 28 | 23% |
| Biochemistry, Genetics and Molecular Biology | 19 | 16% |
| Immunology and Microbiology | 9 | 8% |
| Medicine and Dentistry | 7 | 6% |
| Nursing and Health Professions | 2 | 2% |
| Other | 14 | 12% |
| Unknown | 41 | 34% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 May 2018.
All research outputs
#15,508,366
of 23,047,237 outputs
Outputs from Microbial Ecology
#1,478
of 2,070 outputs
Outputs of similar age
#208,964
of 327,927 outputs
Outputs of similar age from Microbial Ecology
#29
of 37 outputs
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