| Title |
Characterization and multi-generational stability of the growth hormone transgene (EO-1α) responsible for enhanced growth rates in Atlantic Salmon
|
|---|---|
| Published in |
Transgenic Research, August 2006
|
| DOI | 10.1007/s11248-006-0020-5 |
| Pubmed ID | |
| Authors |
Edward S. Yaskowiak, Margaret A. Shears, Alka Agarwal-Mawal, Garth L. Fletcher |
| Abstract |
Transgenic technologies provide a promising means by which desirable traits can be introduced into cultured fish species within a single generation thus accelerating the production of genetically superior broodstock for aquaculture. However, before such fish are allowed to be marketed as food they must receive government regulatory approval. Two pivotal regulatory requirements are: (1) complete characterization of the genomically integrated transgene and, (2) demonstration that the transgene remains stable over multiple generations. We have generated a stable line of growth hormone (GH) transgenic Atlantic salmon (Salmo salar) using an "all fish" gene construct (opAFP-GHc2) containing a growth hormone cDNA from chinook salmon whose expression is regulated by the 5' promoter and 3' termination regions derived from an ocean pout antifreeze protein (AFP) gene. In this study we show that a reorganized form of the opAFP-GHc2 construct (termed EO-1alpha) integrated as a single functional copy into a 35 bp repeat region of the genomic DNA. PCR based mapping revealed that the linear sequence of the EO-1alpha integrant was organized as follows: base pairs 1580-2193 of the ocean pout promoter region followed by the intact chinook salmon GH cDNA, the complete ocean pout antifreeze 3' region, and the first 1678 bp of the ocean pout antifreeze 5' region. Sequence analysis of the EO-1alpha integrant and genomic flanking regions in F2 and F4 generation salmon revealed that they were identical. In addition, apart from the disruption at the integration sites, the consensus sequences of the integrant in these two generations of salmon were identical to the sequence of the opAFP-GHc2 construct. These results indicate that the EO-1alpha transgene codes for the chinook salmon GH, and that the transgene and the integration site have remained stable over multiple generations. |
Mendeley readers
The data shown below were compiled from readership statistics for 82 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 1% |
| Sweden | 1 | 1% |
| Uganda | 1 | 1% |
| Unknown | 79 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 26 | 32% |
| Researcher | 10 | 12% |
| Student > Master | 8 | 10% |
| Student > Ph. D. Student | 7 | 9% |
| Professor > Associate Professor | 4 | 5% |
| Other | 8 | 10% |
| Unknown | 19 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 31 | 38% |
| Biochemistry, Genetics and Molecular Biology | 11 | 13% |
| Environmental Science | 3 | 4% |
| Unspecified | 2 | 2% |
| Immunology and Microbiology | 2 | 2% |
| Other | 7 | 9% |
| Unknown | 26 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 57. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 May 2022.
All research outputs
#621,261
of 22,653,392 outputs
Outputs from Transgenic Research
#9
of 889 outputs
Outputs of similar age
#813
of 65,326 outputs
Outputs of similar age from Transgenic Research
#1
of 10 outputs
Altmetric has tracked 22,653,392 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 889 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one has done particularly well, scoring higher than 98% of its peers.
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