| Title |
MicroRNA-375 functions as a tumor suppressor in osteosarcoma by targeting PIK3CA
|
|---|---|
| Published in |
Tumor Biology, June 2015
|
| DOI | 10.1007/s13277-015-3614-9 |
| Pubmed ID | |
| Authors |
Zhi-cai Shi, Xue-rong Chu, Yun-gang Wu, Jin-hui Wu, Chun-wen Lu, Run-xiao Lü, Mu-chen Ding, Ning-fang Mao |
| Abstract |
Osteosarcoma has become one of the most common primary malignant bone tumors in childhood and adult. Numerous studies have demonstrated that aberrant microRNA (miRNA) expression is involved in human disease including cancer. To date, the potential miRNAs regulating osteosarcoma growth and progression are not fully identified yet. Herein, we showed that miR-375 was frequently downregulated in osteosarcoma tissue and cell lines compared to normal human colon tissues. Overexpression of miR-375 resulted in decreased expression of PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha) at both mRNA and protein levels. We found that miR-375 overexpression markedly suppressed cell proliferation in vitro. And inhibition of miR-375 promotes osteosarcoma growth. Mechanistic studies showed that PIK3CA was a potential target of miR-375 and it mediated reduction of PIK3CA resulted in suppression of PI3K/Akt pathway. Taken together, our results demonstrate that miR-375 functions as a growth-suppressive miRNA and plays an important role in inhibiting the tumorigenesis through targeting PIK3CA in osteosarcoma. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 25 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 25 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 8 | 32% |
| Researcher | 4 | 16% |
| Student > Ph. D. Student | 2 | 8% |
| Lecturer | 1 | 4% |
| Student > Doctoral Student | 1 | 4% |
| Other | 3 | 12% |
| Unknown | 6 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 40% |
| Medicine and Dentistry | 4 | 16% |
| Agricultural and Biological Sciences | 2 | 8% |
| Immunology and Microbiology | 2 | 8% |
| Chemistry | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 6 | 24% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 June 2015.
All research outputs
#20,276,249
of 22,808,725 outputs
Outputs from Tumor Biology
#1,834
of 2,622 outputs
Outputs of similar age
#223,197
of 267,100 outputs
Outputs of similar age from Tumor Biology
#101
of 156 outputs
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