| Title |
Determination of Synacthen® in dried blood spots for doping control analysis using liquid chromatography tandem mass spectrometry
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, April 2015
|
| DOI | 10.1007/s00216-015-8674-6 |
| Pubmed ID | |
| Authors |
Laura Tretzel, Andreas Thomas, Hans Geyer, Philippe Delahaut, Wilhelm Schänzer, Mario Thevis |
| Abstract |
Dried blood spot (DBS) sampling, a technique used for taking whole blood samples dried on a filter paper, was initially reported in 1963 by Robert Guthrie. While the diagnostic analysis of metabolic disorders in newborns was the focus of investigations at that time, the number of established applications for preclinical drug development, toxicological studies, and therapeutic drug monitoring increased enormously in the last decades. As a consequence of speed, simplicity, and minimal invasiveness, DBS recommends itself as the preferential technique in sports drug testing. The present approach highlights for the first time the development of a screening assay for the analysis of the synthetic human adrenocorticotropic hormone tetracosactide hexaacetate (Synacthen(®)) in DBS using liquid chromatography tandem mass spectrometry. Highly purified sample extracts were obtained by an advanced sample preparation procedure including the addition of an internal standard (d8-tetracosactide) and immunoaffinity purification. The method's overall recovery was 27.6 %, and the assay's imprecision was calculated between 8.1 and 17.9 % for intraday and 12.9 to 20.5 % for interday measurements. Stability of the synthetic peptide in DBS was shown for at least 10 days at room temperature and presents a major benefit, since a rapid degradation in conventionally applied matrices such as urine or plasma is well known. With a limit of detection of 50 pg/mL, a detection window of several hours is expected considering reported steady-state plasma levels of 300 pg/mL after intramuscular application of Synacthen(®) Depot (1 mg). The analysis of authentic DBS samples within the scope of an administration study with 250 μg Synacthen(®) (short stimulation test) demonstrated the great potential of the developed assay to simplify the analysis of Synacthen(®) for doping control purposes. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 35 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 35 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 10 | 29% |
| Student > Ph. D. Student | 5 | 14% |
| Researcher | 3 | 9% |
| Student > Postgraduate | 2 | 6% |
| Student > Bachelor | 1 | 3% |
| Other | 6 | 17% |
| Unknown | 8 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 10 | 29% |
| Pharmacology, Toxicology and Pharmaceutical Science | 6 | 17% |
| Agricultural and Biological Sciences | 2 | 6% |
| Sports and Recreations | 2 | 6% |
| Nursing and Health Professions | 1 | 3% |
| Other | 5 | 14% |
| Unknown | 9 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 June 2015.
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#22,758,309
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Outputs from Analytical & Bioanalytical Chemistry
#7,541
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Outputs of similar age
#240,422
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Outputs of similar age from Analytical & Bioanalytical Chemistry
#101
of 174 outputs
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