| Title |
Biochemical Adaptations in Zea mays Roots to Short-Term Pb2+ Exposure: ROS Generation and Metabolism
|
|---|---|
| Published in |
Bulletin of Environmental Contamination and Toxicology, June 2015
|
| DOI | 10.1007/s00128-015-1564-y |
| Pubmed ID | |
| Authors |
Gurpreet Kaur, Shubhpreet Kaur, Harminder Pal Singh, Daizy Rani Batish, Ravinder Kumar Kohli, Valbha Rishi |
| Abstract |
The present study investigated the effect of lead (0, 16, 40 and 80 mg L(-1) Pb(2+)) exposure for 3, 12 and 24 h on root biochemistry in hydroponically grown Zea mays (maize). Pb(2+) exposure (80 mg L(-1)) enhanced malondialdehyde content (239 %-427 %), reactive carbonyl groups (425 %-512 %) and H2O2 (129 %-294 %) accumulation during 3-24 h of treatment, thereby indicating cellular peroxidation and oxidative damage. The quantitative estimations were in accordance with in situ detection of ROS generation (using 2',7'-dichlorodihydrofluorescein diacetate dye) and H2O2 accumulation. Pb(2+) treatment significantly reduced ascorbate and glutathione content during 3-24 h of exposure. On the contrary, levels of non-protein thiols were enhanced by 3-11.8 time over control in response to 16-80 mg L(-1) Pb(2+) treatment, after 24 h. A dose-dependent induction in ascorbate peroxidase and lipoxygenase enzyme activity was observed in Z. mays roots. The activities of ascorbate-recycling enzymes (dehydroascorbate reductase and monodehydroascorbate reductase) were significantly increased in relation to concentration and duration of Pb(2+) treatment. The study concludes that Pb(2+)-exposure induces ROS-mediated oxidative damage during early period of exposure despite the upregulation of enzymes of ascorbate-glutathione cycle. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 6% |
| Unknown | 16 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 18% |
| Student > Doctoral Student | 2 | 12% |
| Unspecified | 1 | 6% |
| Other | 1 | 6% |
| Professor > Associate Professor | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 9 | 53% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 35% |
| Biochemistry, Genetics and Molecular Biology | 1 | 6% |
| Unspecified | 1 | 6% |
| Unknown | 9 | 53% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 February 2016.
All research outputs
#16,371,088
of 24,119,703 outputs
Outputs from Bulletin of Environmental Contamination and Toxicology
#2,634
of 4,112 outputs
Outputs of similar age
#160,793
of 270,544 outputs
Outputs of similar age from Bulletin of Environmental Contamination and Toxicology
#16
of 56 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,112 research outputs from this source. They receive a mean Attention Score of 3.0. This one is in the 33rd percentile – i.e., 33% of its peers scored the same or lower than it.
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We're also able to compare this research output to 56 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 64% of its contemporaries.