Title |
Specific humoral response of hosts with variable schistosomiasis susceptibility
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Published in |
Immunology & Cell Biology, July 2015
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DOI | 10.1038/icb.2015.61 |
Pubmed ID | |
Authors |
Patrick Driguez, Hamish EG McWilliam, Soraya Gaze, David Piedrafita, Mark S Pearson, Rie Nakajima, Mary Duke, Angela Trieu, Denise L Doolan, Fernanda C Cardoso, Algis Jasinskas, Geoffrey N Gobert, Philip L Felgner, Alex Loukas, Els Meeusen, Donald P McManus |
Abstract |
The schistosome blood flukes are one of the largest global causes of parasitic morbidity. Further study of the specific antibody response during schistosomiasis may yield the vaccines and diagnostics needed to combat this disease. Therefore, for the purposes of antigen discovery, sera and antibody secreting cell (ASC) probes from semi-permissive rats and sera from susceptible mice were used to screen a schistosome protein microarray. Following Schistosoma japonicum infection, rats had reduced pathology, increased antibody responses and broader antigen recognition profiles compared with mice. With successive infections, rat global serological reactivity and the number of recognized antigens increased. The local antibody response in rat skin and lung, measured with ASC probes, increased after parasite migration and contributed antigen-specific antibodies to the multivalent serological response. In addition, the temporal variation of anti-parasite serum antibodies after infection and reinfection followed patterns that appear related to the antigen driving the response. Among the 29 antigens differentially recognized by the infected hosts, were numerous known vaccine candidates, drug targets, and several S. japonicum homologues of human schistosomiasis resistance markers-the tegument allergen-like (TAL) proteins. From this set we prioritized eight proteins that may prove to be novel schistosome vaccine and diagnostic antigens.Immunology and Cell Biology accepted article preview online, 05 June 2015. doi:10.1038/icb.2015.61. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Australia | 2 | 40% |
United Kingdom | 1 | 20% |
Unknown | 2 | 40% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 5 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Australia | 1 | 4% |
Unknown | 22 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 22% |
Researcher | 5 | 22% |
Student > Doctoral Student | 3 | 13% |
Student > Master | 3 | 13% |
Other | 2 | 9% |
Other | 3 | 13% |
Unknown | 2 | 9% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 4 | 17% |
Immunology and Microbiology | 3 | 13% |
Agricultural and Biological Sciences | 3 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Mathematics | 1 | 4% |
Other | 2 | 9% |
Unknown | 9 | 39% |