| Title |
Expression of porcine Mx1 with FMDV IRES enhances the antiviral activity against foot-and-mouth disease virus in PK-15 cells
|
|---|---|
| Published in |
Archives of Virology, June 2015
|
| DOI | 10.1007/s00705-015-2473-4 |
| Pubmed ID | |
| Authors |
Bing Yuan, Hui Fang, Chao Shen, Congyi Zheng |
| Abstract |
Foot-and-mouth disease virus (FMDV) is the most contagious pathogen in cloven-hoofed (two-toed) animals. Due to the rapid replication and spread of FMDV, novel therapeutic strategies are greatly needed to reduce or block FMDV shedding in cases of disease outbreak. Here, we generated an IRES-Mx1 construct in which the internal ribosome entry site (IRES) of FMDV was inserted between the promoter and open reading frame (ORF) of porcine myxovirus resistance protein 1 (poMx1). This construct provides more powerful protection against FMDV infection than the IRES-IFN construct that was previously generated by our group. The results indicate that this IRES-Mx1 construct was able to express poMx1 12 h after transfection and induce a robust immune response. In contrast to the control, the proliferation of virus in transfected cells was significantly inhibited, as evaluated by morphology monitoring, real-time RT-PCR, virus titration and Western blot. In addition, we also found that the antiviral activity in cells transfected with pc-IRES-Mx1 was abolished when the JAK/STAT pathway was repressed, which indicates that the antiviral mechanism of poMx1 is JAK/STAT pathway dependent. Taken together, our data suggest that the antiviral activity of poMx1 is possibly produced by affecting the host cells themselves, instead of interacting with the virus directly. The new construct reported here could be used as a novel effective therapy against FMDV infection. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 13% |
| Researcher | 2 | 13% |
| Student > Ph. D. Student | 1 | 7% |
| Other | 1 | 7% |
| Student > Bachelor | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 7 | 47% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 3 | 20% |
| Biochemistry, Genetics and Molecular Biology | 2 | 13% |
| Veterinary Science and Veterinary Medicine | 1 | 7% |
| Agricultural and Biological Sciences | 1 | 7% |
| Chemistry | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 7 | 47% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 June 2015.
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#20,276,249
of 22,808,725 outputs
Outputs from Archives of Virology
#3,387
of 4,155 outputs
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#222,455
of 266,356 outputs
Outputs of similar age from Archives of Virology
#39
of 67 outputs
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