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X Demographics
Mendeley readers
Attention Score in Context
| Title |
p62/SQSTM1 but not LC3 is accumulated in sarcopenic muscle of mice
|
|---|---|
| Published in |
Journal of Cachexia, Sarcopenia and Muscle, June 2015
|
| DOI | 10.1002/jcsm.12045 |
| Pubmed ID | |
| Authors |
Kunihiro Sakuma, Masakazu Kinoshita, Yoshinori Ito, Miki Aizawa, Wataru Aoi, Akihiko Yamaguchi |
| Abstract |
We investigated the pathway of autophagy signaling linked to sarcopenia of mice. Young adult (3-month) and aged (24- month) C57BL/6J mice were used. Using real-time PCR, Western blotting, and immunohistochemical microscopy, we evaluated the amounts of p62/SQSTM1, LC3, and Beclin-1 in the quadriceps muscle change with aging in mice. Marked fiber atrophy (30%) and many fibers with central nuclei were observed in the aged mice. Western blotting using homogenate of the cytosolic fraction clearly showed that the amounts of p62/SQSTM1 and Beclin-1 proteins were significantly increased in the aged skeletal muscle. The amounts of these proteins in both nuclear and membrane fractions did not change significantly with age. Immunofluorescence labeling indicated that aged mice more frequently possessed p62/SQSTM1-positive fibers in the cytosol in quadriceps muscle than young ones (aged: 14% vs. young: 1%). In aged muscle, p62/SQSTM1-positive fibers were significantly smaller than the surrounding p62/SQSTM1-negative fibers. Aging did not elicit significant changes in the mRNA levels of p62/SQSTM1 and Beclin-1, but decreased LC3 mRNA level. In aged muscle, the location of p62/SQSTM1 immunoreactivity was similar to that of Beclin-1 protein, but not LC3. Sarcopenia in mice appears to include a marked defect of autophagy signaling. |
X Demographics
The data shown below were collected from the profiles of 10 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 5 | 50% |
| Unknown | 5 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 9 | 90% |
| Scientists | 1 | 10% |
Mendeley readers
The data shown below were compiled from readership statistics for 87 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Unknown | 86 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 22 | 25% |
| Student > Bachelor | 11 | 13% |
| Student > Master | 9 | 10% |
| Researcher | 8 | 9% |
| Student > Doctoral Student | 4 | 5% |
| Other | 15 | 17% |
| Unknown | 18 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 28 | 32% |
| Medicine and Dentistry | 15 | 17% |
| Agricultural and Biological Sciences | 12 | 14% |
| Neuroscience | 4 | 5% |
| Nursing and Health Professions | 3 | 3% |
| Other | 5 | 6% |
| Unknown | 20 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 February 2021.
All research outputs
#2,613,478
of 25,658,139 outputs
Outputs from Journal of Cachexia, Sarcopenia and Muscle
#321
of 1,298 outputs
Outputs of similar age
#32,150
of 280,529 outputs
Outputs of similar age from Journal of Cachexia, Sarcopenia and Muscle
#5
of 23 outputs
Altmetric has tracked 25,658,139 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,298 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 16.3. This one has done well, scoring higher than 75% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 280,529 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 88% of its contemporaries.
We're also able to compare this research output to 23 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.