Title |
CTCF/cohesin-binding sites are frequently mutated in cancer
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Published in |
Nature Genetics, June 2015
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DOI | 10.1038/ng.3335 |
Pubmed ID | |
Authors |
Riku Katainen, Kashyap Dave, Esa Pitkänen, Kimmo Palin, Teemu Kivioja, Niko Välimäki, Alexandra E Gylfe, Heikki Ristolainen, Ulrika A Hänninen, Tatiana Cajuso, Johanna Kondelin, Tomas Tanskanen, Jukka-Pekka Mecklin, Heikki Järvinen, Laura Renkonen-Sinisalo, Anna Lepistö, Eevi Kaasinen, Outi Kilpivaara, Sari Tuupanen, Martin Enge, Jussi Taipale, Lauri A Aaltonen |
Abstract |
Cohesin is present in almost all active enhancer regions, where it is associated with transcription factors. Cohesin frequently colocalizes with CTCF (CCCTC-binding factor), affecting genomic stability, expression and epigenetic homeostasis. Cohesin subunits are mutated in cancer, but CTCF/cohesin-binding sites (CBSs) in DNA have not been examined for mutations. Here we report frequent mutations at CBSs in cancers displaying a mutational signature where mutations in A•T base pairs predominate. Integration of whole-genome sequencing data from 213 colorectal cancer (CRC) samples and chromatin immunoprecipitation sequencing (ChIP-exo) data identified frequent point mutations at CBSs. In contrast, CRCs showing an ultramutator phenotype caused by defects in the exonuclease domain of DNA polymerase ɛ (POLE) displayed significantly fewer mutations at and adjacent to CBSs. Analysis of public data showed that multiple cancer types accumulate CBS mutations. CBSs are a major mutational hotspot in the noncoding cancer genome. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 17 | 32% |
United Kingdom | 4 | 8% |
Finland | 3 | 6% |
Canada | 3 | 6% |
Australia | 3 | 6% |
New Zealand | 1 | 2% |
Belgium | 1 | 2% |
Austria | 1 | 2% |
Spain | 1 | 2% |
Other | 3 | 6% |
Unknown | 16 | 30% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 27 | 51% |
Members of the public | 22 | 42% |
Science communicators (journalists, bloggers, editors) | 3 | 6% |
Practitioners (doctors, other healthcare professionals) | 1 | 2% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 7 | 1% |
United States | 5 | <1% |
France | 4 | <1% |
Germany | 2 | <1% |
Italy | 1 | <1% |
Lithuania | 1 | <1% |
Finland | 1 | <1% |
Hungary | 1 | <1% |
Canada | 1 | <1% |
Other | 3 | <1% |
Unknown | 535 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 161 | 29% |
Researcher | 101 | 18% |
Student > Master | 65 | 12% |
Student > Bachelor | 55 | 10% |
Student > Doctoral Student | 34 | 6% |
Other | 64 | 11% |
Unknown | 81 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 208 | 37% |
Agricultural and Biological Sciences | 185 | 33% |
Medicine and Dentistry | 32 | 6% |
Computer Science | 17 | 3% |
Physics and Astronomy | 7 | 1% |
Other | 28 | 5% |
Unknown | 84 | 15% |