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Effect of Storage Temperature on Structure and Function of Cultured Human Oral Keratinocytes

Overview of attention for article published in PLOS ONE, June 2015
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (89th percentile)
  • High Attention Score compared to outputs of the same age and source (87th percentile)

Mentioned by

news
2 news outlets

Citations

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9 Dimensions

Readers on

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22 Mendeley
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Title
Effect of Storage Temperature on Structure and Function of Cultured Human Oral Keratinocytes
Published in
PLOS ONE, June 2015
DOI 10.1371/journal.pone.0128306
Pubmed ID
Authors

Rakibul Islam, Catherine Jackson, Jon R. Eidet, Edward B. Messelt, Rima Maria Corraya, Torstein Lyberg, May Griffith, Darlene A. Dartt, Tor P. Utheim

Abstract

To assess the effect of storage temperature on the viability, phenotype, metabolism, and morphology of cultured human oral keratinocytes (HOK). Cultured HOK cells were stored in HEPES- and sodium bicarbonate-buffered Minimum Essential Medium (MEM) at nine temperatures in approximately 4°C increments from 4°C to 37°C for seven days. Cells were characterized for viability by calcein fluorescence, phenotype retention by immunocytochemistry, metabolic parameters (pH, glucose, lactate, and O2) within the storage medium by blood gas analysis, and morphology by scanning electron microscopy and light microscopy. Relative to the cultured, but non-stored control cells, a high percentage of viable cells were retained only in the 12°C and 16°C storage groups (85%±13% and 68%±10%, respectively). Expression of ABCG2, Bmi1, C/EBPδ, PCNA, cytokeratin 18, and caspase-3 were preserved after storage in the 5 groups between 4°C and 20°C, compared to the non-stored control. Glucose, pH and pO2 in the storage medium declined, whereas lactate increased with increasing storage temperature. Morphology was best preserved following storage of the three groups between 12°C, 16°C, and 20°C. We conclude that storage temperatures of 12°C and 16°C were optimal for maintenance of cell viability, phenotype, and morphology of cultured HOK. The storage method described in the present study may be applicable for other cell types and tissues; thus its significance may extend beyond HOK and the field of ophthalmology.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Korea, Republic of 1 5%
Unknown 21 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 23%
Student > Bachelor 3 14%
Researcher 2 9%
Student > Postgraduate 2 9%
Student > Master 2 9%
Other 4 18%
Unknown 4 18%
Readers by discipline Count As %
Medicine and Dentistry 7 32%
Biochemistry, Genetics and Molecular Biology 3 14%
Agricultural and Biological Sciences 3 14%
Immunology and Microbiology 2 9%
Arts and Humanities 1 5%
Other 2 9%
Unknown 4 18%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 14. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 September 2015.
All research outputs
#2,134,903
of 22,811,321 outputs
Outputs from PLOS ONE
#27,213
of 194,663 outputs
Outputs of similar age
#28,949
of 266,096 outputs
Outputs of similar age from PLOS ONE
#866
of 6,781 outputs
Altmetric has tracked 22,811,321 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 90th percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 194,663 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 15.1. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 266,096 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 89% of its contemporaries.
We're also able to compare this research output to 6,781 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.