Title |
Patterned Delivery of Immunoglobulins to Surfaces Using Microfluidic Networks
|
---|---|
Published in |
Science, May 1997
|
DOI | 10.1126/science.276.5313.779 |
Pubmed ID | |
Authors |
Emmanuel Delamarche, André Bernard, Heinz Schmid, Bruno Michel, Hans Biebuyck |
Abstract |
Microfluidic networks (microFNs) were used to pattern biomolecules with high resolution on a variety of substrates (gold, glass, or polystyrene). Elastomeric microFNs localized chemical reactions between the biomolecules and the surface, requiring only microliters of reagent to cover square millimeter-sized areas. The networks were designed to ensure stability and filling of the microFN and allowed a homogeneous distribution and robust attachment of material to the substrate along the conduits in the microFN. Immunoglobulins patterned on substrates by means of microFNs remained strictly confined to areas enclosed by the network with submicron resolution and were viable for subsequent use in assays. The approach is simple and general enough to suggest a practical way to incorporate biological material on technological substrates. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 5 | 2% |
France | 3 | 1% |
Netherlands | 2 | <1% |
Switzerland | 2 | <1% |
Germany | 2 | <1% |
Canada | 2 | <1% |
United Kingdom | 1 | <1% |
Belgium | 1 | <1% |
Austria | 1 | <1% |
Other | 0 | 0% |
Unknown | 250 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 72 | 27% |
Researcher | 50 | 19% |
Student > Master | 40 | 15% |
Professor | 23 | 9% |
Student > Bachelor | 17 | 6% |
Other | 45 | 17% |
Unknown | 22 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 88 | 33% |
Agricultural and Biological Sciences | 41 | 15% |
Chemistry | 39 | 14% |
Physics and Astronomy | 20 | 7% |
Materials Science | 18 | 7% |
Other | 31 | 12% |
Unknown | 32 | 12% |