Title |
Lysine Acetylation Targets Protein Complexes and Co-Regulates Major Cellular Functions
|
---|---|
Published in |
Science, July 2009
|
DOI | 10.1126/science.1175371 |
Pubmed ID | |
Authors |
Chunaram Choudhary, Chanchal Kumar, Florian Gnad, Michael L. Nielsen, Michael Rehman, Tobias C. Walther, Jesper V. Olsen, Matthias Mann |
Abstract |
Lysine acetylation is a reversible posttranslational modification of proteins and plays a key role in regulating gene expression. Technological limitations have so far prevented a global analysis of lysine acetylation's cellular roles. We used high-resolution mass spectrometry to identify 3600 lysine acetylation sites on 1750 proteins and quantified acetylation changes in response to the deacetylase inhibitors suberoylanilide hydroxamic acid and MS-275. Lysine acetylation preferentially targets large macromolecular complexes involved in diverse cellular processes, such as chromatin remodeling, cell cycle, splicing, nuclear transport, and actin nucleation. Acetylation impaired phosphorylation-dependent interactions of 14-3-3 and regulated the yeast cyclin-dependent kinase Cdc28. Our data demonstrate that the regulatory scope of lysine acetylation is broad and comparable with that of other major posttranslational modifications. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 36 | 1% |
United Kingdom | 19 | <1% |
Germany | 15 | <1% |
France | 10 | <1% |
Netherlands | 6 | <1% |
Japan | 6 | <1% |
Canada | 6 | <1% |
Argentina | 5 | <1% |
Spain | 4 | <1% |
Other | 47 | 2% |
Unknown | 2563 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 712 | 26% |
Researcher | 517 | 19% |
Student > Master | 280 | 10% |
Student > Bachelor | 266 | 10% |
Professor > Associate Professor | 124 | 5% |
Other | 377 | 14% |
Unknown | 441 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 975 | 36% |
Biochemistry, Genetics and Molecular Biology | 670 | 25% |
Chemistry | 188 | 7% |
Medicine and Dentistry | 141 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 51 | 2% |
Other | 194 | 7% |
Unknown | 498 | 18% |