| Title |
Transgenic rabbit production with simian immunodeficiency virus-derived lentiviral vector
|
|---|---|
| Published in |
Transgenic Research, January 2010
|
| DOI | 10.1007/s11248-009-9356-y |
| Pubmed ID | |
| Authors |
L. Hiripi, D. Negre, F.-L. Cosset, K. Kvell, T. Czömpöly, M. Baranyi, E. Gócza, O. Hoffmann, B. Bender, Zs. Bősze |
| Abstract |
Transgenic rabbit is the preferred disease model of atherosclerosis, lipoprotein metabolism and cardiovascular diseases since upon introducing genetic mutations of human genes, rabbit models reflect human physiological and pathological states more accurately than mouse models. Beyond that, transgenic rabbits are also used as bioreactors to produce pharmaceutical proteins in their milk. Since in the laboratory rabbit the conventional transgenesis has worked with the same low efficiency in the last twenty five years and truly pluripotent embryonic stem cells are not available to perform targeted mutagenesis, our aim was to adapt lentiviral transgenesis to this species. A simian immunodeficiency virus based replication defective lentiviral vector was used to create transgenic rabbit through perivitelline space injection of fertilized oocytes. The enhanced green fluorescent protein (GFP) gene was placed under the ubiquitous CAG promoter. Transgenic founder rabbits showed mosaic pattern of GFP expression. Transgene integration and expression was revealed in tissues derived from all three primary germ layers. Transgene expression was detected in the developing sperm cells and could get through the germ line without epigenetic silencing, albeit with very low frequency. Our data show for the first time, that lentiviral transgenesis could be a feasible and viable alternative method to create genetically modified laboratory rabbit. |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 3% |
| Unknown | 32 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 24% |
| Student > Ph. D. Student | 8 | 24% |
| Student > Master | 4 | 12% |
| Student > Bachelor | 4 | 12% |
| Student > Postgraduate | 2 | 6% |
| Other | 2 | 6% |
| Unknown | 5 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 48% |
| Biochemistry, Genetics and Molecular Biology | 5 | 15% |
| Neuroscience | 2 | 6% |
| Arts and Humanities | 1 | 3% |
| Veterinary Science and Veterinary Medicine | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 6 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 January 2010.
All research outputs
#5,827,451
of 22,655,397 outputs
Outputs from Transgenic Research
#302
of 889 outputs
Outputs of similar age
#38,469
of 164,440 outputs
Outputs of similar age from Transgenic Research
#3
of 8 outputs
Altmetric has tracked 22,655,397 research outputs across all sources so far. This one has received more attention than most of these and is in the 74th percentile.
So far Altmetric has tracked 889 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one has gotten more attention than average, scoring higher than 65% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 164,440 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 5 of them.