cDNA Libraries

Chapter 1 Isolation of Fungal Infection Structures from Plant Tissue by Flow Cytometry for Cell-Specific Transcriptome Analysis

Chapter 2 Preparation of a High-Quality cDNA Library from a Single-Cell Quantity of mRNA Using Chum-RNA

Chapter 3 Construction of a Full-Length cDNA Library from Castor Endosperm for High-Throughput Functional Screening

Chapter 4 Full-Length Transcriptome Analysis Using a Bias-Free cDNA Library Prepared with the Vector-Capping Method

Chapter 5 Construction of Improved Yeast Two-Hybrid Libraries

Chapter 6 Normalization of Full-Length-Enriched cDNA

Chapter 7 Bioinformatic Methods for Finding Differentially Expressed Genes in cDNA Libraries, Applied to the Identification of Tumour Vascular Targets

Chapter 8 Enzymatic Production of RNAi Libraries from cDNAs and High-Throughput Selection of Effective shRNA Expression Constructs

Chapter 9 Construction of Small RNA cDNA Libraries for High-Throughput Sequencing

Chapter 10 Focusing Mutations Within Random Libraries to Distinct Areas: Protein Domain Library Generation by Overlap Extension

Chapter 11 Generation of Families of Construct Variants Using Golden Gate Shuffling

Chapter 12 Application of Full-Length cDNA Resources to Gain-of-Function Technology for Characterization of Plant Gene Function

Chapter 13 Construction of Yeast Surface-Displayed cDNA Libraries

Chapter 14 Identification of Protein/Target Molecule Interactions Using Yeast Surface-Displayed cDNA Libraries

Chapter 15 SNP Discovery by Transcriptome Pyrosequencing

Chapter 16 RNA-Seq Analysis of Gene Expression and Alternative Splicing by Double-Random Priming Strategy

Chapter 17 Generation of a Large Catalog of Unique Transcripts for Whole-Genome Expression Analysis in Nonmodel Species