Protein NMR Techniques

Chapter 1 A Novel Bacterial Expression Method with Optimized Parameters for Very High Yield Production of Triple-Labeled Proteins

Chapter 2 Isotopic Labeling of Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis

Chapter 3 Isotope Labeling in Insect Cells

Chapter 4 Isotope Labeling in Mammalian Cells

Chapter 5 Cell-Free Protein Production for NMR Studies

Chapter 6 Cell-Free Membrane Protein Expression for Solid-State NMR

Chapter 7 Expression and Purification of Src-family Kinases for Solution NMR Studies

Chapter 8 NMR Studies of Large Protein Systems

Chapter 9 Protein Dynamics by 15 N Nuclear Magnetic Relaxation

Chapter 10 Bacterial Production and Solution NMR Studies of a Viral Membrane Ion Channel

Chapter 11 Preparation of the Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy

Chapter 12 NMR Studies of Protein–RNA Interactions

Chapter 13 Preparation and Optimization of Protein–DNA Complexes Suitable for Detailed NMR Studies

Chapter 14 NMR Studies of Protein–Ligand Interactions

Chapter 15 In-Cell NMR Spectroscopy in Escherichia coli

Chapter 16 Deuterated Peptides and Proteins: Structure and Dynamics Studies by MAS Solid-State NMR

Chapter 17 Solid-State NMR Spectroscopy of Protein Complexes

Chapter 18 Synthesis, Purification, and Characterization of Single Helix Membrane Peptides and Proteins for NMR Spectroscopy

Chapter 19 Assignment of Backbone Resonances in a Eukaryotic Protein Kinase – ERK2 as a Representative Example

Chapter 20 Electrostatics of Hydrogen Exchange for Analyzing Protein Flexibility

Chapter 21 Fast Protein Backbone NMR Resonance Assignment Using the BATCH Strategy

Chapter 22 Comprehensive Automation for NMR Structure Determination of Proteins

Chapter 23 ARIA for Solution and Solid-State NMR

Chapter 24 Determining Protein Dynamics from 15 N Relaxation Data by Using DYNAMICS