| Title |
Multistep tandem mass spectrometry for Sequencing Cyclic Peptides in an Ion-Trap Mass Spectrometer
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, August 1999
|
| DOI | 10.1016/s1044-0305(99)00049-5 |
| Pubmed ID | |
| Authors |
Lambert C. M. Ngoka, Michael L. Gross |
| Abstract |
Collisionally activated decomposition (CAD) of a protonated cyclic peptide produces a superposition spectrum consisting of fragments produced following random ring opening of the cyclic peptide to give a set of acylium ions (or isomeric equivalents) of the same m/z. Assignment of the correct sequence is often difficult owing to lack of selectivity in the ring opening. A method is presented that utilizes multiple stages of CAD experiments in an electrospray ion-trap mass spectrometer to sequence cyclic peptides. A primary acylium ion is selected from the primary product-ion spectrum and subjected to several stages of CAD. Amino-acid residues are sequentially removed, one at each stage of the CAD, from the C-terminus, until a b2 ion is reached. Results are presented for seven cyclic peptides, ranging in sizes from four to eight amino-acid residues. This method of sequencing cyclic peptides eliminates ambiguities encountered with other MS/MS approaches. The power of the strategy lies in the capability to execute several stages of CAD upon a precursor ion and its decomposition products, allowing the cyclic peptide to be sequenced in an unambiguous, stepwise manner. |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Poland | 1 | 2% |
| France | 1 | 2% |
| Unknown | 39 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 32% |
| Researcher | 6 | 15% |
| Student > Master | 4 | 10% |
| Professor | 3 | 7% |
| Professor > Associate Professor | 2 | 5% |
| Other | 5 | 12% |
| Unknown | 8 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 18 | 44% |
| Agricultural and Biological Sciences | 9 | 22% |
| Biochemistry, Genetics and Molecular Biology | 2 | 5% |
| Immunology and Microbiology | 1 | 2% |
| Medicine and Dentistry | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 9 | 22% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 November 2002.
All research outputs
#8,535,472
of 25,374,647 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#1,226
of 3,834 outputs
Outputs of similar age
#11,275
of 34,663 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#3
of 9 outputs
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So far Altmetric has tracked 3,834 research outputs from this source. They receive a mean Attention Score of 3.8. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
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