| Title |
Intrafollicular thyroid hormone staining in whole-mount zebrafish (Danio rerio) embryos for the detection of thyroid hormone synthesis disruption
|
|---|---|
| Published in |
Fish Physiology and Biochemistry, March 2018
|
| DOI | 10.1007/s10695-018-0488-y |
| Pubmed ID | |
| Authors |
Kristina Rehberger, Lisa Baumann, Markus Hecker, Thomas Braunbeck |
| Abstract |
Endocrine-disrupting chemicals are known to impact multiple hormonal axes of vertebrates, among which the thyroid system is crucial for multiple developmental and physiological processes. Thus, the present study focused on the semi-quantitative visualization of intrafollicular triiodothyronine (T3) and thyroxin (T4) in zebrafish embryos as a potential test system for the detection of disrupted thyroid hormone synthesis. To this end, an antibody-based fluorescence double-staining protocol for whole-mount zebrafish embryos and larvae was adapted to simultaneously detect intrafollicular T3 and T4. During normal development until 10 days post-fertilization (dpf), the number of thyroid follicles increased along the ventral aorta. Concentrations of T4 and T3, measured by fluorescence intensity, increased until 6 dpf, but decreased thereafter. Exposure of zebrafish embryos to propylthiouracil (PTU), a known inhibitor of TH synthesis, resulted in a significant decrease in the number of follicles that stained for T3, whereas a trend for increase in follicles that stained for T4 was observed. In contrast, fluorescence intensity for both thyroid hormones decreased significantly after exposure to PTU. Overall, the zebrafish embryo appears to be suitable for the simultaneous visualization and detection of changing intrafollicular TH contents during normal development and after PTU treatment. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 41 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 7 | 17% |
| Student > Ph. D. Student | 6 | 15% |
| Student > Bachelor | 5 | 12% |
| Researcher | 5 | 12% |
| Student > Doctoral Student | 3 | 7% |
| Other | 1 | 2% |
| Unknown | 14 | 34% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 20% |
| Agricultural and Biological Sciences | 5 | 12% |
| Environmental Science | 3 | 7% |
| Neuroscience | 2 | 5% |
| Nursing and Health Professions | 1 | 2% |
| Other | 2 | 5% |
| Unknown | 20 | 49% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 December 2022.
All research outputs
#7,148,724
of 23,342,092 outputs
Outputs from Fish Physiology and Biochemistry
#61
of 873 outputs
Outputs of similar age
#124,867
of 333,384 outputs
Outputs of similar age from Fish Physiology and Biochemistry
#1
of 37 outputs
Altmetric has tracked 23,342,092 research outputs across all sources so far. This one has received more attention than most of these and is in the 68th percentile.
So far Altmetric has tracked 873 research outputs from this source. They receive a mean Attention Score of 1.7. This one has done particularly well, scoring higher than 92% of its peers.
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We're also able to compare this research output to 37 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.