| Title |
How to prove the existence of metabolons?
|
|---|---|
| Published in |
Phytochemistry Reviews, April 2017
|
| DOI | 10.1007/s11101-017-9509-1 |
| Pubmed ID | |
| Authors |
Jean-Etienne Bassard, Barbara Ann Halkier |
| Abstract |
Sequential enzymes in biosynthetic pathways are organized in metabolons. It is challenging to provide experimental evidence for the existence of metabolons as biosynthetic pathways are composed of highly dynamic protein-protein interactions. Many different methods are being applied, each with strengths and weaknesses. We will present and evaluate several techniques that have been applied in providing evidence for the orchestration of the biosynthetic pathways of cyanogenic glucosides and glucosinolates in metabolons. These evolutionarily related pathways have ER-localized cytochromes P450 that are proposed to function as anchoring site for assembly of the enzymes into metabolons. Additionally, we have included commonly used techniques, even though they have not been used (yet) on these two pathways. In the review, special attention will be given to less-exploited fluorescence-based methods such as FCS and FLIM. Ultimately, understanding the orchestration of biosynthetic pathways may contribute to successful engineering in heterologous hosts. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 82 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 82 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 22 | 27% |
| Researcher | 12 | 15% |
| Student > Bachelor | 7 | 9% |
| Student > Master | 7 | 9% |
| Other | 6 | 7% |
| Other | 15 | 18% |
| Unknown | 13 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 32 | 39% |
| Agricultural and Biological Sciences | 22 | 27% |
| Chemistry | 3 | 4% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 2% |
| Environmental Science | 2 | 2% |
| Other | 5 | 6% |
| Unknown | 16 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 May 2018.
All research outputs
#20,492,220
of 23,055,429 outputs
Outputs from Phytochemistry Reviews
#308
of 334 outputs
Outputs of similar age
#269,611
of 309,962 outputs
Outputs of similar age from Phytochemistry Reviews
#8
of 8 outputs
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