| Title |
Xpert MTB/RIF diagnosis of childhood tuberculosis from sputum and stool samples in a high TB-HIV-prevalent setting
|
|---|---|
| Published in |
European Journal of Clinical Microbiology & Infectious Diseases, May 2018
|
| DOI | 10.1007/s10096-018-3272-0 |
| Pubmed ID | |
| Authors |
Patrick Orikiriza, Margaret Nansumba, Dan Nyehangane, Mathieu Bastard, Ivan Taremwa Mugisha, Denis Nansera, Juliet Mwanga-Amumpaire, Yap Boum, Elias Kumbakumba, Maryline Bonnet |
| Abstract |
The Xpert MTB/RIF assay is a major advance for diagnosis of tuberculosis (TB) in high-burden countries but is limited in children by their difficulty to produce sputum. We investigated TB in sputum and stool from children with the aim of improving paediatric TB diagnosis. A prospective cohort of children with presumptive TB, provided two sputum or induced sputum at enrolment in a regional referral hospital in Uganda. Stool was collected from those started on TB treatment. All specimen were tested for Xpert MTB/RIF, mycobacteria growth indicator tube (MGIT), Lowenstein Jensen cultures and microscopy (except stool). We compared TB detection between age categories and assessed the performance of Xpert MTB/RIF in sputum and stool. Of the 392 children enrolled, 357 (91.1%) produced at least one sputum sample. Sputum culture yield was 13/357 (3.6%): 3/109 (2.6%), 3/89 (3.2%), 3/101 (2.6%) and 4/44 (8.2%) among children of < 2, 2-5, ≥ 5-10 and > 10 years, respectively (p = 0.599). Xpert MTB/RIF yield was 14/350 (4.0%): 3/104 (2.9%), 4/92 (4.3%), 3/88 (2.9%) and 4/50 (.0%), respectively (p = 0.283). Sensitivity and specificity of Xpert MTB/RIF in sputum against sputum culture were 90.9% (95% CI 58.7-99.8) and 99.1% (99.1-99.8). In stool, it was 55.6% (21.2-86.3) and 98.2% (98.2-100) against Xpert MTB/RIF and culture in sputum. Only a minority of children had microbiologically confirmed TB with a higher proportion in children above 10 years. Although sensitivity of Xpert MTB/RIF in stool was low, with good optimization, it might be a good alternative to sputum in children. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 25% |
| United States | 1 | 25% |
| Unknown | 2 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 76 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 76 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 14% |
| Student > Master | 9 | 12% |
| Other | 6 | 8% |
| Student > Doctoral Student | 5 | 7% |
| Student > Ph. D. Student | 3 | 4% |
| Other | 14 | 18% |
| Unknown | 28 | 37% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 22 | 29% |
| Biochemistry, Genetics and Molecular Biology | 5 | 7% |
| Immunology and Microbiology | 5 | 7% |
| Nursing and Health Professions | 3 | 4% |
| Business, Management and Accounting | 2 | 3% |
| Other | 8 | 11% |
| Unknown | 31 | 41% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 November 2023.
All research outputs
#6,641,433
of 25,081,505 outputs
Outputs from European Journal of Clinical Microbiology & Infectious Diseases
#674
of 2,972 outputs
Outputs of similar age
#106,294
of 333,968 outputs
Outputs of similar age from European Journal of Clinical Microbiology & Infectious Diseases
#7
of 46 outputs
Altmetric has tracked 25,081,505 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 2,972 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.7. This one has done well, scoring higher than 77% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 333,968 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.