Title |
Batrachochytrium dendrobatidis Can Infect and Cause Mortality in the Nematode Caenorhabditiselegans
|
---|---|
Published in |
Mycopathologia, October 2011
|
DOI | 10.1007/s11046-011-9470-2 |
Pubmed ID | |
Authors |
E. J. Shapard, A. S. Moss, M. J. San Francisco |
Abstract |
Batrachochytrium dendrobatidis (Bd) has been identified as a causative agent in the precipitous decline of amphibians worldwide. Studies on the fungus including its ability to infect and kill the host require use of frogs, a precious resource. Therefore, the development of an alternate host model to study the virulence of the fungus would be useful. Here, we show that Bd can cause mortality in the nematode Caenorhabditis elegans. Incubation of Bd with C. elegans resulted in greater than 70% mortality in the nematodes over a period of 24 h. Fluorescence microscopy using propidium iodide, a fluorescent dye used to determine cell viability, and tactile assays were used to discriminate between live and dead nematodes. These observations suggest that C. elegans may be a useful model organism to study the pathogenicity and virulence mechanisms of Bd. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 13% |
Sweden | 1 | 6% |
Portugal | 1 | 6% |
Colombia | 1 | 6% |
Unknown | 11 | 69% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 12 | 75% |
Scientists | 4 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 5 | 6% |
Hungary | 1 | 1% |
Poland | 1 | 1% |
Brazil | 1 | 1% |
Unknown | 71 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 18 | 23% |
Researcher | 15 | 19% |
Student > Master | 13 | 16% |
Student > Bachelor | 9 | 11% |
Professor | 5 | 6% |
Other | 14 | 18% |
Unknown | 5 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 48 | 61% |
Biochemistry, Genetics and Molecular Biology | 8 | 10% |
Environmental Science | 6 | 8% |
Veterinary Science and Veterinary Medicine | 3 | 4% |
Linguistics | 1 | 1% |
Other | 5 | 6% |
Unknown | 8 | 10% |