| Title |
Dynamic nuclear polarization of membrane proteins: covalently bound spin-labels at protein–protein interfaces
|
|---|---|
| Published in |
Journal of Biomolecular NMR, April 2015
|
| DOI | 10.1007/s10858-015-9919-6 |
| Pubmed ID | |
| Authors |
Benjamin J. Wylie, Boris G. Dzikovski, Shane Pawsey, Marc Caporini, Melanie Rosay, Jack H. Freed, Ann E. McDermott |
| Abstract |
We demonstrate that dynamic nuclear polarization of membrane proteins in lipid bilayers may be achieved using a novel polarizing agent: pairs of spin labels covalently bound to a protein of interest interacting at an intermolecular interaction surface. For gramicidin A, nitroxide tags attached to the N-terminal intermolecular interface region become proximal only when bimolecular channels forms in the membrane. We obtained signal enhancements of sixfold for the dimeric protein. The enhancement effect was comparable to that of a doubly tagged sample of gramicidin C, with intramolecular spin pairs. This approach could be a powerful and selective means for signal enhancement in membrane proteins, and for recognizing intermolecular interfaces. |
Mendeley readers
The data shown below were compiled from readership statistics for 53 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Denmark | 1 | 2% |
| France | 1 | 2% |
| Unknown | 50 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 28% |
| Researcher | 13 | 25% |
| Student > Doctoral Student | 4 | 8% |
| Professor | 4 | 8% |
| Student > Master | 4 | 8% |
| Other | 9 | 17% |
| Unknown | 4 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 29 | 55% |
| Biochemistry, Genetics and Molecular Biology | 5 | 9% |
| Agricultural and Biological Sciences | 5 | 9% |
| Business, Management and Accounting | 2 | 4% |
| Physics and Astronomy | 2 | 4% |
| Other | 4 | 8% |
| Unknown | 6 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 September 2019.
All research outputs
#6,420,771
of 22,813,792 outputs
Outputs from Journal of Biomolecular NMR
#107
of 614 outputs
Outputs of similar age
#76,299
of 264,602 outputs
Outputs of similar age from Journal of Biomolecular NMR
#2
of 12 outputs
Altmetric has tracked 22,813,792 research outputs across all sources so far. This one has received more attention than most of these and is in the 70th percentile.
So far Altmetric has tracked 614 research outputs from this source. They receive a mean Attention Score of 2.9. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 264,602 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 70% of its contemporaries.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.