| Title |
Integrated miRNA and mRNA expression profiling of tension force-induced bone formation in periodontal ligament cells
|
|---|---|
| Published in |
In Vitro Cellular & Developmental Biology - Animal, June 2015
|
| DOI | 10.1007/s11626-015-9892-0 |
| Pubmed ID | |
| Authors |
Maolin Chang, Heng Lin, Meng Luo, Jie Wang, Guangli Han |
| Abstract |
Tension force-induced bone formation is a complex biological process altered by various factors, for example miRNAs and gene regulatory network. However, we know little about critical gene regulators and their functional consequences on this complex process. The aim of this study was to determine the integrated relation between microRNA and mRNA expression in tension force-induced bone formation in periodontal ligament cells by a system biological approach. We identified 818 mRNAs and 32 miRNAs differentially expressed between cyclic tension force-stimulated human periodontal ligament cells and control cells by microarrays. By using miRNA/mRNA network analysis, protein-protein interactions network analysis, and hub analysis, we found that miR-195-5p, miR-424-5p, miR-1297, miR-3607-5p, miR-145-5p, miR-4328, and miR-224-5p were core microRNAs of tension force-induced bone formation. WDR33, HSPH1, ERBB3, RIF1, IKBKB, CREB1, FGF2, and PAG1 were identified as hubs of the PPI network, suggesting the biological significance in this process. The miRNA expression was further examined in human PDLC and animal samples by using quantitative real-time PCR. Thus, we proposed a model of tension force-induced bone formation which is co-regulated through integration of the miRNA and mRNA. This study illustrated the benefits of system biological approaches in the analysis of tension force-induced bone formation as a complex biological process. We used public information and our experimental data to do comprehensive analysis and revealed the coordination transcriptional control of miRNAs of tension force-induced bone formation. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 33% |
| United States | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 38 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 16% |
| Student > Ph. D. Student | 5 | 13% |
| Student > Postgraduate | 3 | 8% |
| Student > Master | 3 | 8% |
| Student > Bachelor | 2 | 5% |
| Other | 9 | 24% |
| Unknown | 10 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 14 | 37% |
| Agricultural and Biological Sciences | 5 | 13% |
| Biochemistry, Genetics and Molecular Biology | 4 | 11% |
| Unspecified | 1 | 3% |
| Unknown | 14 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 June 2015.
All research outputs
#17,763,547
of 22,813,792 outputs
Outputs from In Vitro Cellular & Developmental Biology - Animal
#547
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Outputs of similar age
#177,365
of 264,425 outputs
Outputs of similar age from In Vitro Cellular & Developmental Biology - Animal
#8
of 19 outputs
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So far Altmetric has tracked 791 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 26th percentile – i.e., 26% of its peers scored the same or lower than it.
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