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Mendeley readers
Attention Score in Context
| Title |
Stimulation of the cardiac myocyte Na+-K+ pump due to reversal of its constitutive oxidative inhibition
|
|---|---|
| Published in |
American Journal of Physiology: Cell Physiology, June 2015
|
| DOI | 10.1152/ajpcell.00392.2014 |
| Pubmed ID | |
| Authors |
Karin K M Chia, Chia-Chi Liu, Elisha J Hamilton, Alvaro Garcia, Natasha A Fry, William Hannam, Gemma A Figtree, Helge H Rasmussen |
| Abstract |
Protein kinase C can activate NADPH oxidase and induce glutathionylation of the β1 Na(+)-K(+) pump subunit, inhibiting activity of the catalytic α subunit. To examine if signaling of nitric oxide-induced soluble guanylyl cyclase (sGC)/cGMP/protein kinase G can cause Na(+)-K(+) pump stimulation by counteracting PKC/NADPH oxidase-dependent inhibition cardiac myocytes were exposed to Ang II to activate NADPH oxidase and inhibit Na(+)-K(+) pump current (Ip). Co-exposure to 3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole (YC-1) to stimulate sGC prevented the decrease of Ip. Prevention of the decrease was abolished by inhibition of protein phosphatases 2A (PP2A) but not by inhibition of PP1 and it was reproduced by an activator of PP2A. Consistent with a reciprocal relationship between β1 Na(+)-K(+) pump subunit glutathionylation and pump activity, YC-1 decreased Ang II-induced β1 subunit glutathionylation. The decrease induced by YC-1 was abolished by a PP2A inhibitor. YC-1 decreased phosphorylation of the cytosolic p47phox NADPH oxidase subunit and its co-immunoprecipitation with the membranous p22phox subunit and it decreased O2 (●-)-sensitive dihydroethidium fluorescence of myocytes. Addition of recombinant PP2A to myocyte lysate decreased phosphorylation of p47(phox) indicating the subunit could be a substrate for PP2A. Effects of YC-1 to decrease co-immunoprecipitation of p22phox and p47(phox) NADPH oxidase subunits and decrease β1 Na(+)-K(+) pump subunit glutathionylation were reproduced by activation of nitric oxide-dependent receptor signaling. We conclude that sGC activation in cardiac myocytes causes a PP2A-dependent decrease in NADPH oxidase activity and a decrease in β1 pump subunit glutathionylation. This could account for pump stimulation with neurohormonal oxidative stress expected in vivo. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 33% |
| Researcher | 2 | 33% |
| Student > Master | 2 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 50% |
| Computer Science | 1 | 17% |
| Agricultural and Biological Sciences | 1 | 17% |
| Medicine and Dentistry | 1 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 August 2015.
All research outputs
#19,945,185
of 25,374,917 outputs
Outputs from American Journal of Physiology: Cell Physiology
#2,258
of 2,763 outputs
Outputs of similar age
#189,586
of 277,758 outputs
Outputs of similar age from American Journal of Physiology: Cell Physiology
#35
of 45 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,763 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.9. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 277,758 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 26th percentile – i.e., 26% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 45 others from the same source and published within six weeks on either side of this one. This one is in the 8th percentile – i.e., 8% of its contemporaries scored the same or lower than it.