| Title |
Pseudomonas putida—a versatile host for the production of natural products
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, June 2015
|
| DOI | 10.1007/s00253-015-6745-4 |
| Pubmed ID | |
| Authors |
Anita Loeschcke, Stephan Thies |
| Abstract |
The biosynthesis of natural products by heterologous expression of biosynthetic pathways in amenable production strains enables biotechnological access to a variety of valuable compounds by conversion of renewable resources. Pseudomonas putida has emerged as a microbial laboratory work horse, with elaborated techniques for cultivation and genetic manipulation available. Beyond that, this bacterium offers several particular advantages with regard to natural product biosynthesis, notably a versatile intrinsic metabolism with diverse enzymatic capacities as well as an outstanding tolerance to xenobiotics. Therefore, it has been applied for recombinant biosynthesis of several valuable natural products. This review provides an overview of applications of P. putida as a host organism for the recombinant biosynthesis of such natural products, including rhamnolipids, terpenoids, polyketides and non-ribosomal peptides, and other amino acid-derived compounds. The focus is on de novo natural product synthesis from intrinsic building blocks by means of heterologous gene expression and strain engineering. Finally, the future potential of the bacterium as a chassis organism for synthetic microbiology is pointed out. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 493 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Denmark | 3 | <1% |
| United Kingdom | 1 | <1% |
| Indonesia | 1 | <1% |
| Poland | 1 | <1% |
| Serbia | 1 | <1% |
| Unknown | 486 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 92 | 19% |
| Student > Bachelor | 90 | 18% |
| Student > Ph. D. Student | 86 | 17% |
| Researcher | 53 | 11% |
| Student > Doctoral Student | 25 | 5% |
| Other | 36 | 7% |
| Unknown | 111 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 157 | 32% |
| Agricultural and Biological Sciences | 122 | 25% |
| Engineering | 19 | 4% |
| Chemistry | 18 | 4% |
| Immunology and Microbiology | 16 | 3% |
| Other | 39 | 8% |
| Unknown | 122 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 July 2022.
All research outputs
#2,814,296
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#398
of 8,034 outputs
Outputs of similar age
#35,403
of 268,063 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#5
of 161 outputs
Altmetric has tracked 24,119,703 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 95% of its peers.
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We're also able to compare this research output to 161 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.